@article{oai:repo.qst.go.jp:00043007,
 author = {Yatagai, Fumio and Kurobe, Toshihiro and Nohmi, Takehiko and Ichi, Masumura Ken and Tsukada, Teruyo and Yamaguchi, Hirotake and Eguchi-Kasai, Kiyomi and Fukunishi, Nobuhisa and 谷田貝 文夫 and 能美 健彦 and 増村 健一 and 笠井 清美},
 journal = {Environmental and Molecular Mutagenesis},
 month = {},
 note = {The influence of the loss of p53 gene on heavy-ion-induced mutations was examined by constructing a new line of transgenic mice, p53 knockout (p53-/-) gpt delta. In this mouse model, deletions in lambda DNA integrated into the mouse genome are preferentially selected as Spi- phages, which can then be subjected to molecular analysis. Mice were exposed to 10 Gy of whole-body carbon-ion irradiation. The carbon ions were accelerated to 135 MeV/u by the RIKEN Ring Cyclotron. The p53 defect markedly enhanced the Spi- mutant frequency (MF) in the kidneys of mice exposed to C-ion irradiation: the Spi- MF increased 4.4- and 2.8-fold over the background level after irradiation in p53-/- and p53+/+ mice, respectively. There was no significant difference in the background Spi- MF between p53-/- and p53+/+ mice. Sequence analysis of the Spi- mutants indicated that the enhancement of kidney Spi- MF in p53-/- mice was primarily due to an increase in complex or rearranged-type deletions. In contrast to the kidney, the p53 defect had no effect on the Spi- MF in liver: Spi- MF increased 3.0- and 2.7-fold after the irradiation in p53-/- and p53+/+ mice, respectively. Our results suggest that p53 suppresses deletion mutations induced by heavy-ion irradiation in an organ-specific manner.},
 pages = {216--225},
 title = {Heavy-ion-induced mutations in the gpt delta transgenic mouse: Effect of p53 gene knockout},
 volume = {40},
 year = {2002}
}