WEKO3
アイテム
A new method using Enhanced Green Fluorescent Protein (EGFP) to determine grazing rate on live bacterial cells by protists.
https://repo.qst.go.jp/records/42896
https://repo.qst.go.jp/records/42896a1815dc0-880e-4b52-82d1-cd2466cde9de
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2002-06-04 | |||||
| タイトル | ||||||
| タイトル | A new method using Enhanced Green Fluorescent Protein (EGFP) to determine grazing rate on live bacterial cells by protists. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ishii, Nobuyoshi
× Ishii, Nobuyoshi× Takeda, Hiroshi× Doi, Masahiro× Fuma, Shoichi× Miyamoto, Kiriko× Yanagisawa, Kei× Kawabata, Zenichiro× 石井 伸昌× 武田 洋× 土居 雅広× 府馬 正一× 宮本 霧子× 柳澤 啓 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A new method was developed for estimating grazing rate by protists of live bacteria. Bacterial cells (Escherichia coli bearing plasmid pEGFP) expressing enhanced green fluorescent protein (EGFP) were used as a live bacterial tracer. Ciliates (Tetrahymena thermophila) were fed with EGFP-tagged bacterial cells, and the individual cells taken up by the ciliates were detected by the epifluorescence microscopy. The EGFP fluorescence was stable during the storage of samples fixed with glutaraldehyde. Comparison of clearance rates based on the uptake of EGFP-tagged live cells and fluorescently-labeled heat-killed cells suggested that the use of heat-killed cells underestimates the clearance rates. We suggest that EGFP-tagged bacteria are a useful tracer for determining protist bacterivory in culture and aquatic environments. | |||||
| 書誌情報 |
Limnology 巻 3, p. 47-50, 発行日 2002 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1439-8621 | |||||
