{"created":"2023-05-15T14:33:05.165970+00:00","id":42896,"links":{},"metadata":{"_buckets":{"deposit":"df8de23c-147f-47b4-a2db-9e87ff3507e1"},"_deposit":{"created_by":1,"id":"42896","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"42896"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00042896","sets":["1"]},"author_link":["426909","426913","426916","426908","426917","426912","426915","426906","426907","426910","426914","426911","426905"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"50","bibliographicPageStart":"47","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"Limnology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A new method was developed for estimating grazing rate by protists of live bacteria. Bacterial cells (Escherichia coli bearing plasmid pEGFP) expressing enhanced green fluorescent protein (EGFP) were used as a live bacterial tracer. Ciliates (Tetrahymena thermophila) were fed with EGFP-tagged bacterial cells, and the individual cells taken up by the ciliates were detected by the epifluorescence microscopy. The EGFP fluorescence was stable during the storage of samples fixed with glutaraldehyde. Comparison of clearance rates based on the uptake of EGFP-tagged live cells and fluorescently-labeled heat-killed cells suggested that the use of heat-killed cells underestimates the clearance rates. We suggest that EGFP-tagged bacteria are a useful tracer for determining protist bacterivory in culture and aquatic environments.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1439-8621","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishii, Nobuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"426905","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takeda, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"426906","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Doi, Masahiro"}],"nameIdentifiers":[{"nameIdentifier":"426907","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fuma, Shoichi"}],"nameIdentifiers":[{"nameIdentifier":"426908","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyamoto, Kiriko"}],"nameIdentifiers":[{"nameIdentifier":"426909","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yanagisawa, Kei"}],"nameIdentifiers":[{"nameIdentifier":"426910","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawabata, Zenichiro"}],"nameIdentifiers":[{"nameIdentifier":"426911","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井 伸昌","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426912","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"武田 洋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"土居 雅広","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426914","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"府馬 正一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426915","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"宮本 霧子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426916","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"柳澤 啓","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426917","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A new method using Enhanced Green Fluorescent Protein (EGFP) to determine grazing rate on live bacterial cells by protists. ","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A new method using Enhanced Green Fluorescent Protein (EGFP) to determine grazing rate on live bacterial cells by protists. "}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2002-06-04"},"publish_date":"2002-06-04","publish_status":"0","recid":"42896","relation_version_is_last":true,"title":["A new method using Enhanced Green Fluorescent Protein (EGFP) to determine grazing rate on live bacterial cells by protists. "],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:33:40.129045+00:00"}