@article{oai:repo.qst.go.jp:00042896,
 author = {Ishii, Nobuyoshi and Takeda, Hiroshi and Doi, Masahiro and Fuma, Shoichi and Miyamoto, Kiriko and Yanagisawa, Kei and Kawabata, Zenichiro and 石井 伸昌 and 武田 洋 and 土居 雅広 and 府馬 正一 and 宮本 霧子 and 柳澤 啓},
 journal = {Limnology},
 month = {},
 note = {A new method was developed for estimating grazing rate by protists of live bacteria. Bacterial cells (Escherichia coli bearing plasmid pEGFP) expressing enhanced green fluorescent protein (EGFP) were used as a live bacterial tracer. Ciliates (Tetrahymena thermophila) were fed with EGFP-tagged bacterial cells, and the individual cells taken up by the ciliates were detected by the epifluorescence microscopy. The EGFP fluorescence was stable during the storage of samples fixed with glutaraldehyde. Comparison of clearance rates based on the uptake of EGFP-tagged live cells and fluorescently-labeled heat-killed cells suggested that the use of heat-killed cells underestimates the clearance rates. We suggest that EGFP-tagged bacteria are a useful tracer for determining protist bacterivory in culture and aquatic environments.},
 pages = {47--50},
 title = {A new method using Enhanced Green Fluorescent Protein (EGFP) to determine grazing rate on live bacterial cells by protists.},
 volume = {3},
 year = {2002}
}