WEKO3
アイテム
High- and low-LET induced chromosome damage in human lymphocytes: a time-course of aberrations in metaphase and interphase.
https://repo.qst.go.jp/records/42872
https://repo.qst.go.jp/records/42872cea11efa-94d3-413c-a275-aecbfd080bef
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2002-02-12 | |||||
| タイトル | ||||||
| タイトル | High- and low-LET induced chromosome damage in human lymphocytes: a time-course of aberrations in metaphase and interphase. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
George, Kerry
× George, Kerry× Wu, Honglu× Willingham, Velonoca× Furusawa, Yoshiya× Kawata, Tetsuya× A, Cucinotta Francesca× ジョージ ケリー× ウー ホングル× 古澤 佳也× 川田 哲也× クシノッタ フランク |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | PURPOSE: To investigate how cell-cycle delays in human peripheral lymphocytes affect the expression of complex chromosome damage in metaphase following high- and low-LET radiation exposure. MATERIALS AND METHODS: Whole blood was irradiated in vitro with a low and a high dose of 1 GeV u(-1) iron particles, 400MeV u(-1) neon particles or y-rays. Lymphocytes were cultured and metaphase cells were collected at different time points after 48-84h in culture. Interphase chromosomes were prematurely condensed using calyculin-A, either 48 or 72 h after exposure to iron particles or gamma-rays. Cells in first division were analysed using a combination of FISH whole-chromosome painting and DAPI/ Hoechst 33258 harlequin staining. RESULTS: There was a delay in expression of chromosome damage in metaphase that was LET- and dose-dependant. This delay was mostly related to the late emergence of complex-type damage into metaphase. Yields of damage in PCC collected 48 h after irradiation with iron particles were similar to values obtained from cells undergoing mitosis after prolonged incubation. CONCLUSION: The yield of high-LET radiation-induced complex chromosome damage could be underestimated when analysing metaphase cells collected at one time point after irradiation. Chemically induced PCC is a more accurate technique since problems with complicated cell-cycle delays are avoided. | |||||
| 書誌情報 |
International Journal of Radiation Biology 巻 77, p. 175-183, 発行日 2001 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0955-3002 | |||||
