@article{oai:repo.qst.go.jp:00042872,
 author = {George, Kerry and Wu, Honglu and Willingham, Velonoca and Furusawa, Yoshiya and Kawata, Tetsuya and A, Cucinotta Francesca and ジョージ ケリー and ウー ホングル and 古澤 佳也 and 川田 哲也 and クシノッタ フランク},
 journal = {International Journal of Radiation Biology},
 month = {},
 note = {PURPOSE: To investigate how cell-cycle delays in human peripheral lymphocytes affect the expression of complex chromosome damage in metaphase following high- and low-LET radiation exposure. MATERIALS AND METHODS: Whole blood was irradiated in vitro with a low and a high dose of 1 GeV u(-1) iron particles, 400MeV u(-1) neon particles or y-rays. Lymphocytes were cultured and metaphase cells were collected at different time points after 48-84h in culture. Interphase chromosomes were prematurely condensed using calyculin-A, either 48 or 72 h after exposure to iron particles or gamma-rays. Cells in first division were analysed using a combination of FISH whole-chromosome painting and DAPI/ Hoechst 33258 harlequin staining. RESULTS: There was a delay in expression of chromosome damage in metaphase that was LET- and dose-dependant. This delay was mostly related to the late emergence of complex-type damage into metaphase. Yields of damage in PCC collected 48 h after irradiation with iron particles were similar to values obtained from cells undergoing mitosis after prolonged incubation. CONCLUSION: The yield of high-LET radiation-induced complex chromosome damage could be underestimated when analysing metaphase cells collected at one time point after irradiation. Chemically induced PCC is a more accurate technique since problems with complicated cell-cycle delays are avoided.},
 pages = {175--183},
 title = {High- and low-LET induced chromosome damage in human lymphocytes: a time-course of aberrations in metaphase and interphase.},
 volume = {77},
 year = {2001}
}