{"created":"2023-05-15T14:33:04.145824+00:00","id":42872,"links":{},"metadata":{"_buckets":{"deposit":"52cd6d17-a054-4ecd-b5d8-a9ade4805b89"},"_deposit":{"created_by":1,"id":"42872","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"42872"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00042872","sets":["1"]},"author_link":["426736","426732","426729","426734","426730","426739","426738","426731","426735","426733","426737"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"183","bibliographicPageStart":"175","bibliographicVolumeNumber":"77","bibliographic_titles":[{"bibliographic_title":"International Journal of Radiation Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"PURPOSE: To investigate how cell-cycle delays in human peripheral lymphocytes affect the expression of complex chromosome damage in metaphase following high- and low-LET radiation exposure. MATERIALS AND METHODS: Whole blood was irradiated in vitro with a low and a high dose of 1 GeV u(-1) iron particles, 400MeV u(-1) neon particles or y-rays. Lymphocytes were cultured and metaphase cells were collected at different time points after 48-84h in culture. Interphase chromosomes were prematurely condensed using calyculin-A, either 48 or 72 h after exposure to iron particles or gamma-rays. Cells in first division were analysed using a combination of FISH whole-chromosome painting and DAPI/ Hoechst 33258 harlequin staining. RESULTS: There was a delay in expression of chromosome damage in metaphase that was LET- and dose-dependant. This delay was mostly related to the late emergence of complex-type damage into metaphase. Yields of damage in PCC collected 48 h after irradiation with iron particles were similar to values obtained from cells undergoing mitosis after prolonged incubation. CONCLUSION: The yield of high-LET radiation-induced complex chromosome damage could be underestimated when analysing metaphase cells collected at one time point after irradiation. Chemically induced PCC is a more accurate technique since problems with complicated cell-cycle delays are avoided.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0955-3002","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"George, Kerry"}],"nameIdentifiers":[{"nameIdentifier":"426729","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wu, Honglu"}],"nameIdentifiers":[{"nameIdentifier":"426730","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Willingham, Velonoca"}],"nameIdentifiers":[{"nameIdentifier":"426731","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furusawa, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"426732","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawata, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"426733","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"A, Cucinotta Francesca"}],"nameIdentifiers":[{"nameIdentifier":"426734","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ジョージ ケリー","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426735","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ウー ホングル","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426736","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426737","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"川田 哲也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426738","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"クシノッタ フランク","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"426739","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"High- and low-LET induced chromosome damage in human lymphocytes: a time-course of aberrations in metaphase and interphase.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High- and low-LET induced chromosome damage in human lymphocytes: a time-course of aberrations in metaphase and interphase."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2002-02-12"},"publish_date":"2002-02-12","publish_status":"0","recid":"42872","relation_version_is_last":true,"title":["High- and low-LET induced chromosome damage in human lymphocytes: a time-course of aberrations in metaphase and interphase."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:33:56.332475+00:00"}