WEKO3
アイテム
{"_buckets": {"deposit": "9c34c7ac-29c7-4988-ad69-1c29af5cb95f"}, "_deposit": {"created_by": 1, "id": "77497", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "77497"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00077497", "sets": ["28"]}, "author_link": ["805074", "805076", "805075", "805073", "805070", "805069", "805071", "805072"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2019-11-15", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We have treated more than 1300 patients with bone and soft tissue tumors including osteosarcoma (OS), and has achieved relatively good outcome. However, the 5-year survival rate is still less than 40%, therefore we need to develop a new combination therapy for further improving treatment results. In this study, we investigate the cell-killing mechanisms by combination of carbon-ion beam and drugs. OS cell lines, KHOS and U2OS were used. Analyses of cell cycle, apoptosis, ERK/AKT signal pathway were performed after carbon-ion beam alone or in combination with osteoporosis treating drug zoledronic acid (ZOL) or anticancer drug cisplatin (CDDP). we found that carbon-ion beam combined with ZOL significantly suppressed OS cell proliferation by arresting cell cycle, inducing apoptosis compared to X-ray or carbon-ion beam alone. In addition, the combination of carbon-ion beam and ZOL inhibited migration as well as invasion of OS cells, induced phosphorylation of MEK, and significantly inhibited ERK/AKT signal pathway related to cell proliferation, as compared with carbon-ion beam, X-ray irradiation or ZOL alone treatment. On the other hand, carbon-ion beam dose-dependently increased induction of apoptosis, and it was further increased when combined with CDDP. In addition, combined treatment with carbon-ion beam and CDDP significantly suppressed Bcl2 gene expression and further suppressed Becli1 or LC3 gene expression as compared to carbon-ion beam, X-ray irradiation alone, or X-ray irradiation and CDDP combination. Taken together, carbon-ion beam in combination with ZOL or CDDP significantly disrupts OS cells compared to carbon-ion beam alone.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "日本放射線影響学会第62回大会", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sai, Sei"}], "nameIdentifiers": [{"nameIdentifier": "805069", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ho Kim, Eun"}], "nameIdentifiers": [{"nameIdentifier": "805070", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Masao"}], "nameIdentifiers": [{"nameIdentifier": "805071", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimori, Akira"}], "nameIdentifiers": [{"nameIdentifier": "805072", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sai, Sei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "805073", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ho Kim, Eun", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "805074", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Masao", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "805075", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimori, Akira", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "805076", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/77497", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-11-19"}, "publish_date": "2019-11-19", "publish_status": "0", "recid": "77497", "relation": {}, "relation_version_is_last": true, "title": ["Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells"], "weko_shared_id": -1}
Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells
https://repo.qst.go.jp/records/77497
https://repo.qst.go.jp/records/774979955587b-fb8e-46b3-92da-9a2171360fbc
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-11-19 | |||||
タイトル | ||||||
タイトル | Carbon Ion Beam Combined with Drugs Effectively Destroy Osteosarcoma Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Sai, Sei
× Sai, Sei× Ho Kim, Eun× Suzuki, Masao× Fujimori, Akira× Sai, Sei× Ho Kim, Eun× Suzuki, Masao× Fujimori, Akira |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have treated more than 1300 patients with bone and soft tissue tumors including osteosarcoma (OS), and has achieved relatively good outcome. However, the 5-year survival rate is still less than 40%, therefore we need to develop a new combination therapy for further improving treatment results. In this study, we investigate the cell-killing mechanisms by combination of carbon-ion beam and drugs. OS cell lines, KHOS and U2OS were used. Analyses of cell cycle, apoptosis, ERK/AKT signal pathway were performed after carbon-ion beam alone or in combination with osteoporosis treating drug zoledronic acid (ZOL) or anticancer drug cisplatin (CDDP). we found that carbon-ion beam combined with ZOL significantly suppressed OS cell proliferation by arresting cell cycle, inducing apoptosis compared to X-ray or carbon-ion beam alone. In addition, the combination of carbon-ion beam and ZOL inhibited migration as well as invasion of OS cells, induced phosphorylation of MEK, and significantly inhibited ERK/AKT signal pathway related to cell proliferation, as compared with carbon-ion beam, X-ray irradiation or ZOL alone treatment. On the other hand, carbon-ion beam dose-dependently increased induction of apoptosis, and it was further increased when combined with CDDP. In addition, combined treatment with carbon-ion beam and CDDP significantly suppressed Bcl2 gene expression and further suppressed Becli1 or LC3 gene expression as compared to carbon-ion beam, X-ray irradiation alone, or X-ray irradiation and CDDP combination. Taken together, carbon-ion beam in combination with ZOL or CDDP significantly disrupts OS cells compared to carbon-ion beam alone. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 日本放射線影響学会第62回大会 | |||||
発表年月日 | ||||||
日付 | 2019-11-15 | |||||
日付タイプ | Issued |