WEKO3
アイテム
{"_buckets": {"deposit": "3e5a70b9-99de-480b-a8b6-b234c658173d"}, "_deposit": {"created_by": 1, "id": "70860", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "70860"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00070860", "sets": ["28"]}, "author_link": ["696304", "696296", "696297", "696303", "696301", "696306", "696305", "696300", "696294", "696302", "696299", "696298", "696295"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2012-08-31", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "It was reported that reactive oxygen species (ROS) were generated by heating an aqueous solution containing oxygen. The GSH dependent reduction of a nitroxyl radical, TEMPOL, is accelerated temperature dependently. This reaction can be restricted by adding SOD or by deoxygenating the reaction mixture, therefore used as an index of O2- generation. In this presentation, the hyperthermia-induced ROS scavenging ability of rat plasma was tested.\nA reaction mixture containing 0.1 mM nitroxyl radical (TEMPOL) and 1 mM GSH was prepared using 100 mM phosphate buffer (PB) containing 0.05 mM DTPA. The reaction mixture was kept in a screw-top vial, and was incubated in a water bath at 37 or 44[deg]C. The time course of the EPR signal of TEMPOL in the reaction mixture was measured by an X-band EPR spectrometer (JEOL, Tokyo, Japan). The similar experiment was done using rat plasma instead of 100 mM PB. Effect of BSA and/or ascorbic acid was tested.\nROS generation in an aqueous fluid containing oxygen at hyperthermal temperature was demonstrated. Adding 0.5% albumin to the reaction mixture could almost cancel the ROS generation at 37[deg]C; however, 0.5% albumin could not cancel the ROS at 44[deg]C, i.e. hyperthermal temperature. Ascorbic acid above 0.02 mM also could almost cancel ROS at 37[deg]C, however, 0.03 mM could cancel ROS at 44[deg]C.\nHyperthermia induced ROS in rat plasma can be mostly canceled by relatively high concentration of albumin and ascorbic acid in the plasma.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "ICHO \u0026 JCTM 2012 (The 11th International Congress of Hyperthermic Oncology and The 29th Japanese Congress of Thermal Medicine)", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ueno, Megumi"}], "nameIdentifiers": [{"nameIdentifier": "696294", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nyuui, Minako"}], "nameIdentifiers": [{"nameIdentifier": "696295", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakanishi, Ikuo"}], "nameIdentifiers": [{"nameIdentifier": "696296", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Anzai, Kazunori"}], "nameIdentifiers": [{"nameIdentifier": "696297", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ozawa, Toshihiko"}], "nameIdentifiers": [{"nameIdentifier": "696298", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Kenichiro"}], "nameIdentifiers": [{"nameIdentifier": "696299", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Uto, Yoshihiro"}], "nameIdentifiers": [{"nameIdentifier": "696300", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "上野 恵美", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "696301", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "乳井 美奈子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "696302", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "中西 郁夫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "696303", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "安西 和紀", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "696304", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "小澤 俊彦", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "696305", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "松本 謙一郎", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "696306", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Cancelation of Hyperhermia Induced Reactive Oxygen Species in Rat Plasma", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Cancelation of Hyperhermia Induced Reactive Oxygen Species in Rat Plasma"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/70860", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-09-03"}, "publish_date": "2012-09-03", "publish_status": "0", "recid": "70860", "relation": {}, "relation_version_is_last": true, "title": ["Cancelation of Hyperhermia Induced Reactive Oxygen Species in Rat Plasma"], "weko_shared_id": -1}
Cancelation of Hyperhermia Induced Reactive Oxygen Species in Rat Plasma
https://repo.qst.go.jp/records/70860
https://repo.qst.go.jp/records/70860433516a1-42ff-4743-b1ea-682f986e63c4
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2012-09-03 | |||||
タイトル | ||||||
タイトル | Cancelation of Hyperhermia Induced Reactive Oxygen Species in Rat Plasma | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Ueno, Megumi
× Ueno, Megumi× Nyuui, Minako× Nakanishi, Ikuo× Anzai, Kazunori× Ozawa, Toshihiko× Matsumoto, Kenichiro× Uto, Yoshihiro× 上野 恵美× 乳井 美奈子× 中西 郁夫× 安西 和紀× 小澤 俊彦× 松本 謙一郎 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It was reported that reactive oxygen species (ROS) were generated by heating an aqueous solution containing oxygen. The GSH dependent reduction of a nitroxyl radical, TEMPOL, is accelerated temperature dependently. This reaction can be restricted by adding SOD or by deoxygenating the reaction mixture, therefore used as an index of O2- generation. In this presentation, the hyperthermia-induced ROS scavenging ability of rat plasma was tested. A reaction mixture containing 0.1 mM nitroxyl radical (TEMPOL) and 1 mM GSH was prepared using 100 mM phosphate buffer (PB) containing 0.05 mM DTPA. The reaction mixture was kept in a screw-top vial, and was incubated in a water bath at 37 or 44[deg]C. The time course of the EPR signal of TEMPOL in the reaction mixture was measured by an X-band EPR spectrometer (JEOL, Tokyo, Japan). The similar experiment was done using rat plasma instead of 100 mM PB. Effect of BSA and/or ascorbic acid was tested. ROS generation in an aqueous fluid containing oxygen at hyperthermal temperature was demonstrated. Adding 0.5% albumin to the reaction mixture could almost cancel the ROS generation at 37[deg]C; however, 0.5% albumin could not cancel the ROS at 44[deg]C, i.e. hyperthermal temperature. Ascorbic acid above 0.02 mM also could almost cancel ROS at 37[deg]C, however, 0.03 mM could cancel ROS at 44[deg]C. Hyperthermia induced ROS in rat plasma can be mostly canceled by relatively high concentration of albumin and ascorbic acid in the plasma. |
|||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | ICHO & JCTM 2012 (The 11th International Congress of Hyperthermic Oncology and The 29th Japanese Congress of Thermal Medicine) | |||||
発表年月日 | ||||||
日付 | 2012-08-31 | |||||
日付タイプ | Issued |