WEKO3
アイテム
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In this study, we evaluated the radiolabeled human anti-EGFR monoclonal antibody for this purpose.\n\\nMaterial and Methods: High EGFR-expressing HCC cell lines were selected from four cell lines (Hep-G2, HuH-7, SK-Hep1 and Li-7) by western blot analysis and immunohistochemistry. Cancer cells (2x106) were inoculated subcutaneously into BALB/c-nu/nu mice with Matrigel. Anti-EGFR monoclonal antibody (048-006, IgG1 subclass) were labeled with 125I using the chloramine-T or 111In using CHX-A\"-DTPA as a bifunctional chelate. The in vitro character of the radiolabeled 048-006 was evaluated by cell binding, competitive inhibition and internalization assays, and then in vivo character was assessed by biodistribution and planar imaging in tumor model mice. \n\\nResults: Western blotting analysis revealed that HuH-7 showed the highest EGFR expression among four cell lines tested. EGFR was expressed on the cell membrane of HuH-7 by immunohistochemistry. After subcutaneous inoculation with Matrigel, HuH-7 cells successfully formed tumor xenografts showing membranous EGFR expression. In vitro studies showed that 125I-048-006 and 111In-048-006 specifically bound to HuH-7 with the affinity constants of 9.3x108M-1 and 8.0x108M-1, respectively. This antibody was internalized after binding to EGFR. 111In-048-006 highly accumulated in xenografted tumors (highest tumor uptake of 21.24+/-5.84 %ID/gram obtained at 48 hours after injection), and the tumor xenograft was clearly visualized by planar imaging. \n\\nConclusion: This radiolabeled human anti-EGFR monoclonal antibody 048-006 would be promising for the imaging HCC.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "World Federation of Nuclear Medicine and Biology 2010", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sogawa, Chizuru"}], "nameIdentifiers": [{"nameIdentifier": "689836", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuji, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "689837", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furukawa, Takako"}], "nameIdentifiers": [{"nameIdentifier": "689838", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koizumi, Mitsuru"}], "nameIdentifiers": [{"nameIdentifier": "689839", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kurosawa, Yoshikazu"}], "nameIdentifiers": [{"nameIdentifier": "689840", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saga, Tsuneo"}], "nameIdentifiers": [{"nameIdentifier": "689841", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "et.al"}], "nameIdentifiers": [{"nameIdentifier": "689842", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "曽川 千鶴", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "689843", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "辻 厚至", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "689844", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古川 高子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "689845", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "小泉 満", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "689846", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "黒澤 良和", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "689847", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "佐賀 恒夫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "689848", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/70255", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-09-27"}, "publish_date": "2010-09-27", "publish_status": "0", "recid": "70255", "relation": {}, "relation_version_is_last": true, "title": ["Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody"], "weko_shared_id": -1}
Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody
https://repo.qst.go.jp/records/70255
https://repo.qst.go.jp/records/7025569415399-aa0a-4f5e-9acf-a43eb784963b
Item type | 会議発表用資料 / Presentation(1) | |||||
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公開日 | 2010-09-27 | |||||
タイトル | ||||||
タイトル | Molecular Imaging of Hepatocellular Carcinoma using Human Anti-EGFR Monoclonal Antibody | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Sogawa, Chizuru
× Sogawa, Chizuru× Tsuji, Atsushi× Furukawa, Takako× Koizumi, Mitsuru× Kurosawa, Yoshikazu× Saga, Tsuneo× et.al× 曽川 千鶴× 辻 厚至× 古川 高子× 小泉 満× 黒澤 良和× 佐賀 恒夫 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Introduction: Over-expression or mutation of EGFR is identified in several cancers including hepatocellular carcinoma (HCC). We are trying to develop a novel imaging method to detect EGFR over-expression at an early stage of cancers including HCC. In this study, we evaluated the radiolabeled human anti-EGFR monoclonal antibody for this purpose. \nMaterial and Methods: High EGFR-expressing HCC cell lines were selected from four cell lines (Hep-G2, HuH-7, SK-Hep1 and Li-7) by western blot analysis and immunohistochemistry. Cancer cells (2x106) were inoculated subcutaneously into BALB/c-nu/nu mice with Matrigel. Anti-EGFR monoclonal antibody (048-006, IgG1 subclass) were labeled with 125I using the chloramine-T or 111In using CHX-A"-DTPA as a bifunctional chelate. The in vitro character of the radiolabeled 048-006 was evaluated by cell binding, competitive inhibition and internalization assays, and then in vivo character was assessed by biodistribution and planar imaging in tumor model mice. \nResults: Western blotting analysis revealed that HuH-7 showed the highest EGFR expression among four cell lines tested. EGFR was expressed on the cell membrane of HuH-7 by immunohistochemistry. After subcutaneous inoculation with Matrigel, HuH-7 cells successfully formed tumor xenografts showing membranous EGFR expression. In vitro studies showed that 125I-048-006 and 111In-048-006 specifically bound to HuH-7 with the affinity constants of 9.3x108M-1 and 8.0x108M-1, respectively. This antibody was internalized after binding to EGFR. 111In-048-006 highly accumulated in xenografted tumors (highest tumor uptake of 21.24+/-5.84 %ID/gram obtained at 48 hours after injection), and the tumor xenograft was clearly visualized by planar imaging. \nConclusion: This radiolabeled human anti-EGFR monoclonal antibody 048-006 would be promising for the imaging HCC. |
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会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | World Federation of Nuclear Medicine and Biology 2010 | |||||
発表年月日 | ||||||
日付 | 2010-09-23 | |||||
日付タイプ | Issued |