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Here we evaluated *Cu-ATSM as an indicator of intercellular over-reduced states in disorders with mitochondrial dysfunction. Methods: Mitochondrial DNA-less \u0026#61554;0 cells\u0026#61472; and the parental 143B human osteosarcoma cells, and the cybrids carrying mutated mitochondria from patients of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) (2SD cells) and that carrying wild-type one (2SA) were used. Cells were treated under normoxia or hypoxia and 64Cu-ATSM uptake was examined to compare it with levels of biological reductant NADH and NADPH. Results: \u0026#61554;0 206 cells showed 2.7-fold higher 64Cu-ATSM uptake than parent 143B cells under normoxia (P\u003c0.05), whereas the 64Cu-ATSM uptake was not increased under hypoxia. The NADH and NADPH levels showed correlate change to 64Cu-ATSM uptake, but it was not increased under hypoxia. 2SD cells showed 1.5-fold increase in 64Cu-ATSM uptake under normoxia as compared with 2SA, whereas 2SD cells showed no significant increased 64Cu-ATSM uptake under hypoxia, which was correlated with NADH and NADPH levels.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "ISNM 2011 Annual Meeting", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yoshii, Yukie"}], "nameIdentifiers": [{"nameIdentifier": "633665", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoneda, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "633666", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ikawa, Masamichi"}], "nameIdentifiers": [{"nameIdentifier": "633667", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furukawa, Takako"}], "nameIdentifiers": [{"nameIdentifier": "633668", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kiyono, Yashushi"}], "nameIdentifiers": [{"nameIdentifier": "633669", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori, Tetsuya"}], "nameIdentifiers": [{"nameIdentifier": "633670", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshii, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "633671", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oyama, Nobuyuki"}], "nameIdentifiers": [{"nameIdentifier": "633672", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okazawa, Hidehiko"}], "nameIdentifiers": [{"nameIdentifier": "633673", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujibayashi, Yasuhisa"}], "nameIdentifiers": [{"nameIdentifier": "633674", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "吉井 幸恵", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "633675", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "古川 高子", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "633676", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "吉井 裕", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "633677", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "藤林 康久", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "633678", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients."}]}, "item_type_id": "10005", "owner": "1", "path": ["29"], "permalink_uri": "https://repo.qst.go.jp/records/64252", "pubdate": {"attribute_name": "公開日", "attribute_value": "2011-06-13"}, "publish_date": "2011-06-13", "publish_status": "0", "recid": "64252", "relation": {}, "relation_version_is_last": true, "title": ["A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients."], "weko_shared_id": -1}
A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients.
https://repo.qst.go.jp/records/64252
https://repo.qst.go.jp/records/64252ae92e033-394a-45f5-8f30-7b0721887b70
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2011-06-13 | |||||
タイトル | ||||||
タイトル | A novel application of radiolabeled Cu-ATSM as an indicator of intracellular over-reduced states in disorders with mitochondrial dysfunction: studies with cybrids carrying mitochondrial DNA mutation from MELAS patients. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Yoshii, Yukie
× Yoshii, Yukie× Yoneda, Makoto× Ikawa, Masamichi× Furukawa, Takako× Kiyono, Yashushi× Mori, Tetsuya× Yoshii, Hiroshi× Oyama, Nobuyuki× Okazawa, Hidehiko× Fujibayashi, Yasuhisa× 吉井 幸恵× 古川 高子× 吉井 裕× 藤林 康久 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objectives: Radiolabeled Cu-diacetyl-bis (N4-methylthiosemicarbazone) (*Cu-ATSM), including 60/62/64Cu-ATSM, is a potential imaging agent of hypoxic tumors for PET. We have reported that *Cu-ATSM is trapped in tumor cells under intracellular over-reduced states eg. hypoxia. Here we evaluated *Cu-ATSM as an indicator of intercellular over-reduced states in disorders with mitochondrial dysfunction. Methods: Mitochondrial DNA-less 0 cells and the parental 143B human osteosarcoma cells, and the cybrids carrying mutated mitochondria from patients of mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes (MELAS) (2SD cells) and that carrying wild-type one (2SA) were used. Cells were treated under normoxia or hypoxia and 64Cu-ATSM uptake was examined to compare it with levels of biological reductant NADH and NADPH. Results: 0 206 cells showed 2.7-fold higher 64Cu-ATSM uptake than parent 143B cells under normoxia (P<0.05), whereas the 64Cu-ATSM uptake was not increased under hypoxia. The NADH and NADPH levels showed correlate change to 64Cu-ATSM uptake, but it was not increased under hypoxia. 2SD cells showed 1.5-fold increase in 64Cu-ATSM uptake under normoxia as compared with 2SA, whereas 2SD cells showed no significant increased 64Cu-ATSM uptake under hypoxia, which was correlated with NADH and NADPH levels. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | ISNM 2011 Annual Meeting | |||||
発表年月日 | ||||||
日付 | 2011-06-08 | |||||
日付タイプ | Issued |