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Measurement of temperature distribution inside X-ray irradiated cells
https://repo.qst.go.jp/records/85307
https://repo.qst.go.jp/records/85307ff480567-4a6f-4a46-a8f7-4477cd997e12
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-03-16 | |||||
| タイトル | ||||||
| タイトル | Measurement of temperature distribution inside X-ray irradiated cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Masato, Shigekiyo
× Masato, Shigekiyo× Usami, Noriko× Okabe, Kohki× Akinari, Yokoya× Masato, Shigekiyo× Akinari, Yokoya |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It is becoming recognized that cellular temperature depends on the intracellular condition. The heat balance of chemical reactions inside various organelles may change significantly due to external stress, such as ionizing irradiation. In the present study, HeLa cells were investigated a fluorescent chemical probe, Cellular Thermoprobe for Fluorescence Ratio, to determine temperature of each cell after X-ray irradiation of 10 Gy. We used two methods to irradiate the cells with X-rays. The first is to irradiate the entire dish with X-rays. The second method is to limit the irradiation range and irradiate X-rays separately in the irradiated area and the non-irradiated area. After irradiation with X-rays, it was observed by the microscope. The intensity and distribution of the two fluorescences were analyzed using an image analysis software, ImageJ. The temperatures were then determined from the obtained fluorescent ratios based on a calibration curve. The intracellular temperature were significantly different even between cells in same dish. For the irradiated cells, the temperature was more dispersed among the cells and prone to be statically higher than that of the control cells. Fine temperature-distribution over the cell including various organelles will be also reported. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第64回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2021-09-22 | |||||
| 日付タイプ | Issued | |||||
