{"created":"2023-05-15T15:02:58.050943+00:00","id":85307,"links":{},"metadata":{"_buckets":{"deposit":"0ae41a65-a39a-40c5-9b63-1b5cf60d5959"},"_deposit":{"created_by":1,"id":"85307","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"85307"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00085307","sets":["10:29"]},"author_link":["1028289","1028290","1028291","1028287","1028292","1028288"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2021-09-22","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"It is becoming recognized that cellular temperature depends on the intracellular condition. The heat balance of chemical reactions inside various organelles may change significantly due to external stress, such as ionizing irradiation. In the present study, HeLa cells were investigated a fluorescent chemical probe, Cellular Thermoprobe for Fluorescence Ratio, to determine temperature of each cell after X-ray irradiation of 10 Gy. We used two methods to irradiate the cells with X-rays. The first is to irradiate the entire dish with X-rays. The second method is to limit the irradiation range and irradiate X-rays separately in the irradiated area and the non-irradiated area. After irradiation with X-rays, it was observed by the microscope. The intensity and distribution of the two fluorescences were analyzed using an image analysis software, ImageJ. The temperatures were then determined from the obtained fluorescent ratios based on a calibration curve. The intracellular temperature were significantly different even between cells in same dish. For the irradiated cells, the temperature was more dispersed among the cells and prone to be statically higher than that of the control cells. Fine temperature-distribution over the cell including various organelles will be also reported. ","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第64回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Masato, Shigekiyo"}],"nameIdentifiers":[{"nameIdentifier":"1028287","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Usami, Noriko"}],"nameIdentifiers":[{"nameIdentifier":"1028288","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okabe, Kohki"}],"nameIdentifiers":[{"nameIdentifier":"1028289","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akinari, Yokoya"}],"nameIdentifiers":[{"nameIdentifier":"1028290","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masato, Shigekiyo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1028291","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akinari, Yokoya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1028292","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Measurement of temperature distribution inside X-ray irradiated cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Measurement of temperature distribution inside X-ray irradiated cells"}]},"item_type_id":"10005","owner":"1","path":["29"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-16"},"publish_date":"2022-03-16","publish_status":"0","recid":"85307","relation_version_is_last":true,"title":["Measurement of temperature distribution inside X-ray irradiated cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T17:53:29.971002+00:00"}