WEKO3
アイテム
Raman-activated directed evolution of Euglena gracilis with quantum-beam mutagenesis
https://repo.qst.go.jp/records/85293
https://repo.qst.go.jp/records/85293c17f6e62-8089-4046-a74b-5019103534f8
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-03-15 | |||||
| タイトル | ||||||
| タイトル | Raman-activated directed evolution of Euglena gracilis with quantum-beam mutagenesis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
G. de Pablo , J.
× G. de Pablo , J.× Lindley, M.× Hiramatsu, K.× Goda, K.× Katsuya, Sato× Yutaka, Ono× Katsuya, Sato× Yutaka, Ono |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Biomaterials derived from microalgae are promising as a source of biofuels, drugs, cosmetics, and foods. Among different microalgae, Euglena gracilis has attracted attention for biomaterials production due to its high productivity of paramylon, a potential drug for HIV and colon cancer. To enhance the productivity of paramylon in E.gracilis, directed evolution is a promising approach. In general, directed evolution consists of three key steps: mutagenesis, selection, and amplification. For applying it to the enhancement of the paramylon productivity of E.gracilis, mutagenesis and selection steps are challenging because altering nuclear genome of E.gracilis is difficult due to its polyploid nature and no biomarker such as fluorescent dyes is available for quantifying paramylon. In this study, we tackle these difficulties by combining quantum-beam irradiation for mutagenesis and high-throughput Raman-activated cell screening for label-free selection. | |||||
| 書誌情報 |
QST Takasaki Annual Report 2020 p. 88, 発行日 2022-03 |
|||||
