{"created":"2023-05-15T15:02:57.413340+00:00","id":85293,"links":{},"metadata":{"_buckets":{"deposit":"f46b90b9-36a4-482a-9c7c-8c1486f29930"},"_deposit":{"created_by":1,"id":"85293","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"85293"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00085293","sets":["7"]},"author_link":["1028172","1028174","1028171","1028170","1028176","1028173","1028177","1028175"],"item_10004_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"88","bibliographic_titles":[{"bibliographic_title":"QST Takasaki Annual Report 2020"}]}]},"item_10004_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Biomaterials derived from microalgae are promising as a source of biofuels, drugs, cosmetics, and foods. Among different microalgae, Euglena gracilis has attracted attention for biomaterials production due to its high productivity of paramylon, a potential drug for HIV and colon cancer. To enhance the productivity of paramylon in E.gracilis, directed evolution is a promising approach. In general, directed evolution consists of three key steps: mutagenesis, selection, and amplification. For applying it to the enhancement of the paramylon productivity of E.gracilis, mutagenesis and selection steps are challenging because altering nuclear genome of E.gracilis is difficult due to its polyploid nature and no biomarker such as fluorescent dyes is available for quantifying paramylon. In this study, we tackle these difficulties by combining quantum-beam irradiation for mutagenesis and high-throughput Raman-activated cell screening for label-free selection.","subitem_description_type":"Abstract"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"G. de Pablo , J."}],"nameIdentifiers":[{"nameIdentifier":"1028170","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Lindley, M."}],"nameIdentifiers":[{"nameIdentifier":"1028171","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hiramatsu, K."}],"nameIdentifiers":[{"nameIdentifier":"1028172","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Goda, K."}],"nameIdentifiers":[{"nameIdentifier":"1028173","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsuya, Sato"}],"nameIdentifiers":[{"nameIdentifier":"1028174","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yutaka, Ono"}],"nameIdentifiers":[{"nameIdentifier":"1028175","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Katsuya, Sato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1028176","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yutaka, Ono","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1028177","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Raman-activated directed evolution of Euglena gracilis  with quantum-beam mutagenesis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Raman-activated directed evolution of Euglena gracilis  with quantum-beam mutagenesis"}]},"item_type_id":"10004","owner":"1","path":["7"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-03-15"},"publish_date":"2022-03-15","publish_status":"0","recid":"85293","relation_version_is_last":true,"title":["Raman-activated directed evolution of Euglena gracilis  with quantum-beam mutagenesis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T17:53:51.279981+00:00"}