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Development of Hyperpolarized Molecular Probes for in vivo Detection of Peptidase Activities
https://repo.qst.go.jp/records/84942
https://repo.qst.go.jp/records/849429a595930-5c61-49c9-b8ca-7fc88196a293
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2022-02-01 | |||||
| タイトル | ||||||
| タイトル | Development of Hyperpolarized Molecular Probes for in vivo Detection of Peptidase Activities | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yutaro, Saito
× Yutaro, Saito× Hiroyuki, Yatabe× Iori, Tamura× Ryo, Ishida× Yohei, Kondo× Tomohiro, Seki× Akihiro, Eguchi× Yoichi, Takakusagi× Kazutoshi, Yamamoto× C. Murali, Krishna× Shinsuke, Sando× Yoichi, Takakusagi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Detecting activities of peptidases in vivo is an attractive challenge in chemical biology because peptidase-activities are correlated strongly with various biological phenomena and diseases. Nuclear magnetic resonance (NMR) is a powerful technique for this purpose due to its advantages in high tissue penetration and low invasiveness, allowing a molecular detection at deep area. However, the sensitivity of NMR is severely low for in vivo application. To overcome this problem, dynamic nuclear polarization (DNP) has emerged. NMR signals are dramatically enhanced by using this method. However, there are few molecular probes to detect peptidase-activity by using DNP technique to date. Herein, we have developed a practical molecular probe for DNP-NMR to detect the activity of Aminopeptidase N (APN), which is a representative peptidase, in vivo through rational molecular design. It is demonstrated that this probe has many advantages for in vivo application and enables detection of APN-activity in xenograft tumors bearing mice. Starting with this success, we developed some DNP-NMR molecular probes for detecting peptidase-activities based on chemical design. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PACIFICHEM2021 | |||||
| 発表年月日 | ||||||
| 日付 | 2021-12-16 | |||||
| 日付タイプ | Issued | |||||
