@misc{oai:repo.qst.go.jp:00084942,
 author = {Yutaro, Saito and Hiroyuki, Yatabe and Iori, Tamura and Ryo, Ishida and Yohei, Kondo and Tomohiro, Seki and Akihiro, Eguchi and Yoichi, Takakusagi and Kazutoshi, Yamamoto and C. Murali, Krishna and Shinsuke, Sando and Yoichi, Takakusagi},
 month = {Dec},
 note = {Detecting activities of peptidases in vivo is an attractive challenge in chemical biology because peptidase-activities are correlated strongly with various biological phenomena and diseases. Nuclear magnetic resonance (NMR) is a powerful technique for this purpose due to its advantages in high tissue penetration and low invasiveness, allowing a molecular detection at deep area. However, the sensitivity of NMR is severely low for in vivo application. To overcome this problem, dynamic nuclear polarization (DNP) has emerged. NMR signals are dramatically enhanced by using this method. However, there are few molecular probes to detect peptidase-activity by using DNP technique to date. Herein, we have developed a practical molecular probe for DNP-NMR to detect the activity of Aminopeptidase N (APN), which is a representative peptidase, in vivo through rational molecular design. It is demonstrated that this probe has many advantages for in vivo application and enables detection of APN-activity in xenograft tumors bearing mice. Starting with this success, we developed some DNP-NMR molecular probes for detecting peptidase-activities based on chemical design., PACIFICHEM2021},
 title = {Development of Hyperpolarized Molecular Probes for in vivo Detection of Peptidase Activities},
 year = {2021}
}