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Robust Mutants Isolated through Heavy-Ion Beam Irradiation and Endurance Screening in the Green Alga Haematococcus pluvialis
https://repo.qst.go.jp/records/84805
https://repo.qst.go.jp/records/84805008e4ef5-ce83-4958-b4d1-b21b53c0c927
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-12-24 | |||||
| タイトル | ||||||
| タイトル | Robust Mutants Isolated through Heavy-Ion Beam Irradiation and Endurance Screening in the Green Alga Haematococcus pluvialis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takeshita, Tsuyoshi
× Takeshita, Tsuyoshi× Takita, Kaori× Kotaro, Ishii× Kazama, Yusuke× Abe, Tomoko× Kawano, Shigeyuki× Kotaro, Ishii |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Heavy-ion beams have been widely utilized as a novel and effective mutagen for mutation breeding in diverse species, including algae, but a preferred mutant cannot be easily obtained without a suitable large-scale screening method. We devised a unique, convenient, and effective method for screening mutants of Haematococcus pluvialis to isolate a strain resistant to environmental stress with low white fluorescence, i.e., a robust strain. Haematococcus was irradiated with heavy-ion beams of carbon ions, argon ions, and iron ions at various doses, after which approximately 10,000 surviving colonies were inoculated into 96-well plates, cultured for approximately 2 weeks, and then left to dry in a refrigerator for 3–12 months without a lid. In these unattended 96-well plates, cells in approximately one-third of the wells died and became white, and the remaining wells were approximately evenly split between red and green. The robustness of wild-type and mutant strains isolated from red and green wells was compared under severe environmental-stress conditions (125 µmol photons m−2 s−1, continuous light period, 45 mM sodium acetate). In the wild-type strain, most cells died, and 93.9% of cells emitted white autofluorescence. In contrast, few G4 cells emitted white autofluorescence, indicating a survival rate of 91.8%. Strains with excellent carotenoid production, such as G7 and R1, showed greater robustness compared to wild-type strains. | |||||
| 書誌情報 |
Cytologia 巻 86, 号 4, p. 283-289, 発行日 2021-12 |
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| 出版者 | ||||||
| 出版者 | The Japan Mendel Society | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0011-4545 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1508/cytologia.86.283 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/article/cytologia/86/4/86_D-21-00109/_article | |||||
