{"created":"2023-05-15T15:02:32.686579+00:00","id":84805,"links":{},"metadata":{"_buckets":{"deposit":"24170d65-7733-4a1c-b2c9-f464c226a677"},"_deposit":{"created_by":1,"id":"84805","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"84805"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00084805","sets":["1"]},"author_link":["1023263","1023264","1023266","1023261","1023267","1023262","1023265"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"289","bibliographicPageStart":"283","bibliographicVolumeNumber":"86","bibliographic_titles":[{"bibliographic_title":"Cytologia"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Heavy-ion beams have been widely utilized as a novel and effective mutagen for mutation breeding in diverse species, including algae, but a preferred mutant cannot be easily obtained without a suitable large-scale screening method. We devised a unique, convenient, and effective method for screening mutants of Haematococcus pluvialis to isolate a strain resistant to environmental stress with low white fluorescence, i.e., a robust strain. Haematococcus was irradiated with heavy-ion beams of carbon ions, argon ions, and iron ions at various doses, after which approximately 10,000 surviving colonies were inoculated into 96-well plates, cultured for approximately 2 weeks, and then left to dry in a refrigerator for 3–12 months without a lid. In these unattended 96-well plates, cells in approximately one-third of the wells died and became white, and the remaining wells were approximately evenly split between red and green. The robustness of wild-type and mutant strains isolated from red and green wells was compared under severe environmental-stress conditions (125 µmol photons m−2 s−1, continuous light period, 45 mM sodium acetate). In the wild-type strain, most cells died, and 93.9% of cells emitted white autofluorescence. In contrast, few G4 cells emitted white autofluorescence, indicating a survival rate of 91.8%. Strains with excellent carotenoid production, such as G7 and R1, showed greater robustness compared to wild-type strains.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"The Japan Mendel Society"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1508/cytologia.86.283","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/article/cytologia/86/4/86_D-21-00109/_article","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0011-4545","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takeshita, Tsuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"1023261","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takita, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"1023262","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotaro, Ishii"}],"nameIdentifiers":[{"nameIdentifier":"1023263","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kazama, Yusuke"}],"nameIdentifiers":[{"nameIdentifier":"1023264","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Tomoko"}],"nameIdentifiers":[{"nameIdentifier":"1023265","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawano, Shigeyuki"}],"nameIdentifiers":[{"nameIdentifier":"1023266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotaro, Ishii","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1023267","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Robust Mutants Isolated through Heavy-Ion Beam Irradiation and Endurance Screening in the Green Alga Haematococcus pluvialis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Robust Mutants Isolated through Heavy-Ion Beam Irradiation and Endurance Screening in the Green Alga Haematococcus pluvialis"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-12-24"},"publish_date":"2021-12-24","publish_status":"0","recid":"84805","relation_version_is_last":true,"title":["Robust Mutants Isolated through Heavy-Ion Beam Irradiation and Endurance Screening in the Green Alga Haematococcus pluvialis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:11:10.696508+00:00"}