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Re-evaluations of Zr-DFO Complex Coordination Chemistry for the Estimation of Radiochemical Yields and Chelator-to-Antibody Ratios of 89Zr Immune-PET Tracers
https://repo.qst.go.jp/records/84713
https://repo.qst.go.jp/records/84713b75de59a-c13e-4e36-aa07-49ad2e43ebfa
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-08-14 | |||||
| タイトル | ||||||
| タイトル | Re-evaluations of Zr-DFO Complex Coordination Chemistry for the Estimation of Radiochemical Yields and Chelator-to-Antibody Ratios of 89Zr Immune-PET Tracers | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ryota, Imura (University of Tokyo/JFE Engineering)
× Ryota, Imura (University of Tokyo/JFE Engineering)× Hiroyuki, Ida (JFE Enginnering)× Ichiro, Sasaki× Noriko, Ishioka× Shigeki, Watanabe× Ichiro, Sasaki× Noriko, Ishioka× Shigeki, Watanabe |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Deferoxamine B (DFO) is the most widely used chelator for labeling of zirconium-89 (89Zr) to monoclonal antibody (mAb). Despite the remarkable developments of the clinical 89Zr-immuno-PET, chemical species and stability constants of the Zr-DFO complexes remained controversial. The aim of this study was to re-evaluate their stability constants by identifying species of Zr-DFO complexes, and demonstrate that the stability constants can estimate radiochemical yield (RCY) and chelator-to-antibody ratio (CAR). (2) Methods: Zr-DFO species were determined by UV and ESI-MS spectroscopy. Stability constants and speciation of the Zr-DFO complex were re-determined by potentiometric titration. Complexation inhibition of Zr-DFO by residual impurities was investigated by competition titration. (3) Results: Unknown species, ZrHqDFO2, were suc-cessfully detected by nano-ESI-Q-MS analysis. We revealed that a dominant specie under radio-labeling condition (pH 7) was ZrHDFO and its stability constant (logβ111) was 49.1±0.3. Competi-tion titration revealed that residual oxalate inhibits Zr-DFO complex formation. RCYs in different oxalate concentration (0.1 and 0.04 mol/L) were estimated to be 86% and >99%, which was good agreement with reported results (87%, 97%). (4) Conclusion: This study succeeded in obtaining accurate stability constants of Zr-DFO complexes, and estimating RCY and CAR from accurate stability constants established in this study. | |||||
| 書誌情報 |
Molecules 巻 26, 号 16, p. 4977, 発行日 2021-08 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1420-3049 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/molecules26164977 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.mdpi.com/1420-3049/26/16/4977 | |||||
