{"created":"2023-05-15T15:02:28.603931+00:00","id":84713,"links":{},"metadata":{"_buckets":{"deposit":"4e3908cc-f8f3-43c7-84ec-034dc1e88dcf"},"_deposit":{"created_by":1,"id":"84713","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"84713"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00084713","sets":["1"]},"author_link":["1022267","1022269","1022270","1022274","1022272","1022273","1022271","1022268"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"16","bibliographicPageStart":"4977","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Molecules"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Deferoxamine B (DFO) is the most widely used chelator for labeling of zirconium-89 (89Zr) to monoclonal antibody (mAb). Despite the remarkable developments of the clinical 89Zr-immuno-PET, chemical species and stability constants of the Zr-DFO complexes remained controversial. The aim of this study was to re-evaluate their stability constants by identifying species of Zr-DFO complexes, and demonstrate that the stability constants can estimate radiochemical yield (RCY) and chelator-to-antibody ratio (CAR). (2) Methods: Zr-DFO species were determined by UV and ESI-MS spectroscopy. Stability constants and speciation of the Zr-DFO complex were re-determined by potentiometric titration. Complexation inhibition of Zr-DFO by residual impurities was investigated by competition titration. (3) Results: Unknown species, ZrHqDFO2, were suc-cessfully detected by nano-ESI-Q-MS analysis. We revealed that a dominant specie under radio-labeling condition (pH 7) was ZrHDFO and its stability constant (logβ111) was 49.1±0.3. Competi-tion titration revealed that residual oxalate inhibits Zr-DFO complex formation. RCYs in different oxalate concentration (0.1 and 0.04 mol/L) were estimated to be 86% and >99%, which was good agreement with reported results (87%, 97%). (4) Conclusion: This study succeeded in obtaining accurate stability constants of Zr-DFO complexes, and estimating RCY and CAR from accurate stability constants established in this study.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/molecules26164977","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.mdpi.com/1420-3049/26/16/4977","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1420-3049","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ryota, Imura (University of Tokyo/JFE Engineering)"}],"nameIdentifiers":[{"nameIdentifier":"1022267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hiroyuki, Ida (JFE Enginnering)"}],"nameIdentifiers":[{"nameIdentifier":"1022268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichiro, Sasaki"}],"nameIdentifiers":[{"nameIdentifier":"1022269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Noriko, Ishioka"}],"nameIdentifiers":[{"nameIdentifier":"1022270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shigeki, Watanabe"}],"nameIdentifiers":[{"nameIdentifier":"1022271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichiro, Sasaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1022272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Noriko, Ishioka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1022273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shigeki, Watanabe","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1022274","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Re-evaluations of Zr-DFO Complex Coordination Chemistry for the  Estimation of Radiochemical Yields and Chelator-to-Antibody Ratios of 89Zr  Immune-PET Tracers","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Re-evaluations of Zr-DFO Complex Coordination Chemistry for the  Estimation of Radiochemical Yields and Chelator-to-Antibody Ratios of 89Zr  Immune-PET Tracers"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-08-14"},"publish_date":"2021-08-14","publish_status":"0","recid":"84713","relation_version_is_last":true,"title":["Re-evaluations of Zr-DFO Complex Coordination Chemistry for the  Estimation of Radiochemical Yields and Chelator-to-Antibody Ratios of 89Zr  Immune-PET Tracers"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:14:51.705098+00:00"}