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Microscopic Temperature Control Reveals Cooperative Regulation of Actin–Myosin Interaction by Drebrin E
https://repo.qst.go.jp/records/84663
https://repo.qst.go.jp/records/846636bb21156-9953-403b-b979-a78f10362dc6
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-08-02 | |||||
| タイトル | ||||||
| タイトル | Microscopic Temperature Control Reveals Cooperative Regulation of Actin–Myosin Interaction by Drebrin E | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kubota, Hiroaki
× Kubota, Hiroaki× Ogawa, Hiroyuki× Miyazaki, Makito× 石井, 秀弥× 大山, 廣太郎× Kawamura, Yuki× Shin’ichi, Ishiwata× Suzuki, Madoka× Shuya, Ishii× Kotaro, Oyama |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Drebrin E is a regulatory protein of intracellular force produced by actomyosin complexes, that is, myosin molecular motors interacting with actin filaments. The expression level of drebrin E in nerve cells decreases as the animal grows, suggesting its pivotal but unclarified role in neuronal development. Here, by applying the microscopic heat pulse method to actomyosin motility assay, the regulatory mechanism is examined from the room temperature up to 37 °C without a thermal denaturing of proteins. We show that the inhibition of actomyosin motility by drebrin E is eliminated immediately and reversibly during heating and depends on drebrin E concentration. The direct observation of quantum dot-labeled drebrin E implies its stable binding to actin filaments during the heat-induced sliding. Our results suggest that drebrin E allosterically modifies the actin filament structure to regulate cooperatively the actomyosin activity at the maintained in vivo body temperature. | |||||
| 書誌情報 |
Nano Letters 巻 21, 号 22, p. 9526-9533, 発行日 2021-11 |
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| 出版者 | ||||||
| 出版者 | ACS | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1530-6984 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1021/acs.nanolett.1c02955 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://pubs.acs.org/doi/full/10.1021/acs.nanolett.1c02955 | |||||
