{"created":"2023-05-15T15:02:26.355531+00:00","id":84663,"links":{},"metadata":{"_buckets":{"deposit":"cb4f4b00-4f55-4c1e-afff-b533ab378ae6"},"_deposit":{"created_by":1,"id":"84663","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"84663"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00084663","sets":["1"]},"author_link":["1021817","1021819","1021818","1021824","1021822","1021816","1021825","1021823","1021821","1021820"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageEnd":"9533","bibliographicPageStart":"9526","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"Nano Letters"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Drebrin E is a regulatory protein of intracellular force produced by actomyosin complexes, that is, myosin molecular motors interacting with actin filaments. The expression level of drebrin E in nerve cells decreases as the animal grows, suggesting its pivotal but unclarified role in neuronal development. Here, by applying the microscopic heat pulse method to actomyosin motility assay, the regulatory mechanism is examined from the room temperature up to 37 °C without a thermal denaturing of proteins. We show that the inhibition of actomyosin motility by drebrin E is eliminated immediately and reversibly during heating and depends on drebrin E concentration. The direct observation of quantum dot-labeled drebrin E implies its stable binding to actin filaments during the heat-induced sliding. Our results suggest that drebrin E allosterically modifies the actin filament structure to regulate cooperatively the actomyosin activity at the maintained in vivo body temperature.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1021/acs.nanolett.1c02955","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://pubs.acs.org/doi/full/10.1021/acs.nanolett.1c02955","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1530-6984","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kubota, Hiroaki"}],"nameIdentifiers":[{"nameIdentifier":"1021816","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogawa, Hiroyuki"}],"nameIdentifiers":[{"nameIdentifier":"1021817","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyazaki, Makito"}],"nameIdentifiers":[{"nameIdentifier":"1021818","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井, 秀弥"}],"nameIdentifiers":[{"nameIdentifier":"1021819","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大山, 廣太郎"}],"nameIdentifiers":[{"nameIdentifier":"1021820","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawamura, Yuki"}],"nameIdentifiers":[{"nameIdentifier":"1021821","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shin’ichi, Ishiwata"}],"nameIdentifiers":[{"nameIdentifier":"1021822","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Madoka"}],"nameIdentifiers":[{"nameIdentifier":"1021823","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shuya, Ishii","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1021824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotaro, Oyama","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1021825","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Microscopic Temperature Control Reveals Cooperative Regulation of Actin–Myosin Interaction by Drebrin E","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Microscopic Temperature Control Reveals Cooperative Regulation of Actin–Myosin Interaction by Drebrin E"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2021-08-02"},"publish_date":"2021-08-02","publish_status":"0","recid":"84663","relation_version_is_last":true,"title":["Microscopic Temperature Control Reveals Cooperative Regulation of Actin–Myosin Interaction by Drebrin E"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T18:16:46.991474+00:00"}