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Quantitative comparison of DNA damage by radiation and genotoxic metal compounds using biodosimetric indicators of DNA-damage-induced RNAs and double-strand break in RAW264.7 cells.
https://repo.qst.go.jp/records/82187
https://repo.qst.go.jp/records/821876741330d-f0f4-4377-ba19-a01b559433ae
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-03-16 | |||||
| タイトル | ||||||
| タイトル | Quantitative comparison of DNA damage by radiation and genotoxic metal compounds using biodosimetric indicators of DNA-damage-induced RNAs and double-strand break in RAW264.7 cells. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hiroshi, Ishihara
× Hiroshi, Ishihara× Izumi, Tanaka× Haruko, Yakumaru× Mika, Tanaka× Kazuko, Yokochi× Akihiro, Uehara× Shino, Takeda× Hiroshi, Ishihara× Izumi, Tanaka× Haruko, Yakumaru× Mika, Tanaka× Kazuko, Yokochi× Akihiro, Uehara× Shino, Takeda |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We have previously reported that the levels of DNA damage-induced mRNA and lncRNA in blood cells from mouse irradiated with 10mGy class of low-dose ionizing radiation can be used as biodosimetric quantitative indicators of DNA damage, based on conventional molecular biological technique. The principle of the quantification is the measurement of cellular intermediate RNA products which are generated by known and unknown mechanism triggered by various type of DNA damage. The method is suitable for analysis after single and multiple irradiation in relatively short-time. However, method is not suitable for comparison of damage level followed by hours of continuous irradiation, by the low-dose rate effect. We aimed to establish alternative general method to quantitate DNA damage based on different principles that is applicable for low-dose rate irradiation in heterogenous cell population. Several histochemical procedures were compared using a mouse osteoclastoma cell line, RAW264.7. Among them, the quantitative indicator suitable for low dose irradiation was only gamma H2ax, double-strand break-indicator, and we established the universal methodology to quantify damages in the cell population. Several metallic organic compounds have been known to induced DNA damage. However, quantitative study to compare toxicity level between the compounds and ionizing radiation has not well-investigated. We simultaneously quantitated DNA damage-induced RNAs and gamma-H2ax levels in RAW 264.7 cells after treatment with irradiation or metal compounds. The difference in DNA-damage mechanism between radiation and metal compounds reflects the quantitative values of these indicators. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第63回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2020-10-15 | |||||
| 日付タイプ | Issued | |||||
