@misc{oai:repo.qst.go.jp:00082187,
 author = {Hiroshi, Ishihara and Izumi, Tanaka and Haruko, Yakumaru and Mika, Tanaka and Kazuko, Yokochi and Akihiro, Uehara and Shino, Takeda and Hiroshi, Ishihara and Izumi, Tanaka and Haruko, Yakumaru and Mika, Tanaka and Kazuko, Yokochi and Akihiro, Uehara and Shino, Takeda},
 month = {Oct},
 note = {We have previously reported that the levels of DNA damage-induced mRNA and lncRNA in blood cells from mouse irradiated with 10mGy class of low-dose ionizing radiation can be used as biodosimetric quantitative indicators of DNA damage, based on conventional molecular biological technique.  The principle of the quantification is the measurement of cellular intermediate RNA products which are generated by known and unknown mechanism triggered by various type of DNA damage.  The method is suitable for analysis after single and multiple irradiation in relatively short-time.  However, method is not suitable for comparison of damage level followed by hours of continuous irradiation, by the low-dose rate effect.
  We aimed to establish alternative general method to quantitate DNA damage based on different principles that is applicable for low-dose rate irradiation in heterogenous cell population.  Several histochemical procedures were compared using a mouse osteoclastoma cell line, RAW264.7.  Among them, the quantitative indicator suitable for low dose irradiation was only gamma H2ax, double-strand break-indicator, and we established the universal methodology to quantify damages in the cell population.
  Several metallic organic compounds have been known to induced DNA damage.  However, quantitative study to compare toxicity level between the compounds and ionizing radiation has not well-investigated.  We simultaneously quantitated DNA damage-induced RNAs and gamma-H2ax levels in RAW 264.7 cells after treatment with irradiation or metal compounds.  The difference in DNA-damage mechanism between radiation and metal compounds reflects the quantitative values of these indicators., 日本放射線影響学会第63回大会},
 title = {Quantitative comparison of DNA damage by radiation and genotoxic metal compounds using biodosimetric indicators of DNA-damage-induced RNAs and double-strand break in RAW264.7 cells.},
 year = {2020}
}