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Fluorescence anisotropy study of radiation-induced DNA damage clustering based on FRET
https://repo.qst.go.jp/records/81150
https://repo.qst.go.jp/records/81150f2807e23-d79d-4cb5-953d-269b7cc26a17
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-08-18 | |||||
| タイトル | ||||||
| タイトル | Fluorescence anisotropy study of radiation-induced DNA damage clustering based on FRET | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Akamatsu, Ken
× Akamatsu, Ken× Shikazono, Naoya× Saito, Takeshi× Ken, Akamatsu× Naoya, Shikazono |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | DNA lesion induced by ionizing radiation and chemicals can cause mutation and carcinogenesis. In particular, clustered DNA damage site (cluster), that is a DNA region with multiple lesions within a few helical turns, is believed to hardly be repaired. However, little is known about the structural details of a cluster. We develop a method to characterize a cluster by fluorescence anisotropy measurements based on Förster resonance energy transfer (FRET) using an irradiated plasmid DNA sample labeled with fluorescent dyes on the lesions. We find that proximity of the lesions and/or probability of cluster formation increase with increasing linear energy transfer (LET) of radiation. | |||||
| 書誌情報 |
Analytical and Bioanalytical Chemistry 巻 413, p. 1185-1192, 発行日 2020-11 |
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| 出版者 | ||||||
| 出版者 | Springer | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1618-2642 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s00216-020-03082-w | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://link.springer.com/article/10.1007/s00216-020-03082-w | |||||
