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Flow Cytometry Definition of Rat Mammary Epithelial Cell Populations and Their Distinct Radiation Responses
https://repo.qst.go.jp/records/80333
https://repo.qst.go.jp/records/803336d69a0de-a9d0-408f-9b3d-7b3c7f52f393
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-04-10 | |||||
| タイトル | ||||||
| タイトル | Flow Cytometry Definition of Rat Mammary Epithelial Cell Populations and Their Distinct Radiation Responses | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ken-ichi, Kudo
× Ken-ichi, Kudo× Takabatake, Masaru× Nagata, Kento× Nishimura, Yukiko× Daino, Kazuhiro× Iizuka, Daisuke× Nishimura, Mayumi× Suzuki, Keiji× Kakinuma, Shizuko× Imaoka, Tatsuhiko× Kenichi, Kudo× Masaru, Takabatake× Kento, Nagata× Yukiko, Nishimura× Kazuhiro, Daino× Daisuke, Iizuka× Mayumi, Nishimura× Shizuko, Kakinuma× Tatsuhiko, Imaoka |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Breasts are very susceptible to radiation-induced carcinogenesis, and mammary stem/progenitor cells are potentially important targets of this. The mammary epithelium is maintained as 2 mostly independent lineages of luminal and basal cells. To reveal their immediate radiation responses, we analyzed the mammary glands of female Sprague-Dawley rats, a radiation carcinogenesis model, using colony formation, flow cytometry, and immunofluorescence. The results revealed that flow cytometry successfully fractionates rat mammary cells into CD49fhi CD24lo basal, CD49fmed CD24hi luminal progenitor, and CD49flo CD24hi mature luminal populations, resembling human breast, rather than mouse tissues. The colony-forming ability of the basal cells was more radiosensitive than the luminal progenitor cells. Flow cytometry and immunofluorescence showed more efficient cell cycle arrest, γH2AX responses, and apoptosis in the irradiated luminal progenitor cells than the basal cells. These results provide important insights into the early phase of radiation-induced breast cancer. | |||||
| 書誌情報 |
Radiation Research 巻 194, 号 1, p. 22-37, 発行日 2020-04 |
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| 出版者 | ||||||
| 出版者 | Allen Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0033-7587 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 32352870 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1667/RR15566.1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1667/RR15566.1 | |||||
