@article{oai:repo.qst.go.jp:00080333,
 author = {Ken-ichi, Kudo and Takabatake, Masaru and Nagata, Kento and Nishimura, Yukiko and Daino, Kazuhiro and Iizuka, Daisuke and Nishimura, Mayumi and Suzuki, Keiji and Kakinuma, Shizuko and Imaoka, Tatsuhiko and Kenichi, Kudo and Masaru, Takabatake and Kento, Nagata and Yukiko, Nishimura and Kazuhiro, Daino and Daisuke, Iizuka and Mayumi, Nishimura and Shizuko, Kakinuma and Tatsuhiko, Imaoka},
 issue = {1},
 journal = {Radiation Research},
 month = {Apr},
 note = {Breasts are very susceptible to radiation-induced carcinogenesis, and mammary stem/progenitor cells are potentially important targets of this. The mammary epithelium is maintained as 2 mostly independent lineages of luminal and basal cells. To reveal their immediate radiation responses, we analyzed the mammary glands of female Sprague-Dawley rats, a radiation carcinogenesis model, using colony formation, flow cytometry, and immunofluorescence. The results revealed that flow cytometry successfully fractionates rat mammary cells into CD49fhi CD24lo basal, CD49fmed CD24hi luminal progenitor, and CD49flo CD24hi mature luminal populations, resembling human breast, rather than mouse tissues. The colony-forming ability of the basal cells was more radiosensitive than the luminal progenitor cells. Flow cytometry and immunofluorescence showed more efficient cell cycle arrest, γH2AX responses, and apoptosis in the irradiated luminal progenitor cells than the basal cells. These results provide important insights into the early phase of radiation-induced breast cancer.},
 pages = {22--37},
 title = {Flow Cytometry Definition of Rat Mammary Epithelial Cell Populations and Their Distinct Radiation Responses},
 volume = {194},
 year = {2020}
}