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Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells
https://repo.qst.go.jp/records/80155
https://repo.qst.go.jp/records/801550795419b-377f-40ff-b229-4338145a1de1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Hitomi.S.2020.In.Vivo.34.2437-2441.AscorbatePeroxidase.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-06-08 | |||||
| タイトル | ||||||
| タイトル | Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Hitomi, Suzuro
× Hitomi, Suzuro× Kokabu, Shoichiro× Ken-ichiro, Matsumoto× Shoji, Yoshimi× Ujihara, Izumi× Ono, Kentaro× Matsumoto, Kenichiro× Shoji, Yoshimi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has a greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined the ability of Cyanidioschyzon merolae-derived APX (cAPX) to increase anti-oxidative capacity when expressed in mammalian cells. Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation. Results: Heat and H2O2 stimulation caused ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX. Conclusion: Introduction of cAPX increases anti-oxidative capacity in mammalian cells. | |||||
| 書誌情報 |
in vivo 巻 34, 号 5, p. 2437-2441, 発行日 2020-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0258-851X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.21873/invivo.12058 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://iv.iiarjournals.org/content/34/5/2437.full | |||||
