@article{oai:repo.qst.go.jp:00080155,
 author = {Hitomi, Suzuro and Kokabu, Shoichiro and Ken-ichiro, Matsumoto and Shoji, Yoshimi and Ujihara, Izumi and Ono, Kentaro and Matsumoto, Kenichiro and Shoji, Yoshimi},
 issue = {5},
 journal = {in vivo},
 month = {Sep},
 note = {Background: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has a greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined the ability of Cyanidioschyzon merolae-derived APX (cAPX) to increase anti-oxidative capacity when expressed in mammalian cells. Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H2O2 and acid stimulation. Results: Heat and H2O2 stimulation caused ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H2O2 and acid stimulations than control cells lacking cAPX. Conclusion: Introduction of cAPX increases anti-oxidative capacity in mammalian cells.},
 pages = {2437--2441},
 title = {Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells},
 volume = {34},
 year = {2020}
}