WEKO3
アイテム
Identification of Candidate Genes for Mutated Phenotype by Genome Analysis of Ion-beam-induced Rice Mutants
https://repo.qst.go.jp/records/79277
https://repo.qst.go.jp/records/79277af731108-d2c8-4afd-9614-7972c1ffcab3
| Item type | 一般雑誌記事 / Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-03-10 | |||||
| タイトル | ||||||
| タイトル | Identification of Candidate Genes for Mutated Phenotype by Genome Analysis of Ion-beam-induced Rice Mutants | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ono, Yutaka
× Ono, Yutaka× Ichida, H.× Morita, R.× Nozawa, Shigeki× Sato, Katsuya× Shimizu, A.× Kato, K.× Abe, T.× Hase, Yoshihiro× Ono, Yutaka× Nozawa, Shigeki× Sato, Katsuya× Hase, Yoshihiro |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ion beams are useful mutagens for plant and microbe breeding. They are thought to cause mutations by distinct mechanism from chemical mutagens or gamma rays. To understand the property of induced mutations at a genomic level, we have conducted exome analysis of genomic DNA of rice mutants isolated from a carbon ion-beam-mutagenized population. Five independent rice (cultivar Nipponbare) mutant lines (2 dwarfs and 3 early-heading-date mutants), of which phenotype was confirmed in the M3 generation, were isolated from seed-irradiated popullation (40 Gy of 320-MeV 12C6+ ions). The exome analysis identified a total of 56 mutations. The average number of mutations per line was 11.2 ± 3.3. Among 56 mutations, 6 (1.2 mutations per line on average) were classified as high-impact mutations that cause a frame shift or loss of exons and putativelly generate defective proteins. The identification of a small number of high-impact mutation suggests that it could be easy to detect a causal gene responsible for the mutant phenotype. Indeed, we have found candidate genes likely causing the mutant phenotype in the 4 out of the 5 mutants. |
|||||
| 書誌情報 |
QST Takasaki Annual Report 2018 巻 QST-M-23, p. 73, 発行日 2020-03 |
|||||
