@article{oai:repo.qst.go.jp:00079277,
 author = {Ono, Yutaka and Ichida, H. and Morita, R. and Nozawa, Shigeki and Sato, Katsuya and Shimizu, A. and Kato, K. and Abe, T. and Hase, Yoshihiro and Ono, Yutaka and Nozawa, Shigeki and Sato, Katsuya and Hase, Yoshihiro},
 journal = {QST Takasaki Annual Report 2018},
 month = {Mar},
 note = {Ion beams are useful mutagens for plant and microbe breeding. They are thought to cause mutations by distinct mechanism from chemical mutagens or gamma rays. To understand the property of induced mutations at a genomic level, we have conducted exome analysis of genomic DNA of rice mutants isolated from a carbon ion-beam-mutagenized population. 
Five independent rice (cultivar Nipponbare) mutant lines (2 dwarfs  and 3 early-heading-date mutants), of which phenotype was confirmed in the M3 generation, were isolated from seed-irradiated popullation (40 Gy of 320-MeV 12C6+ ions). The exome analysis identified a total of 56 mutations. The average number of mutations per line was 11.2 ± 3.3. Among 56 mutations, 6 (1.2 mutations per line on average) were classified as high-impact mutations that cause a frame shift or loss of exons and putativelly generate defective proteins. The identification of a small number of high-impact mutation suggests that it could be easy to detect a causal gene responsible for the mutant phenotype. Indeed, we have found candidate genes likely causing the mutant phenotype in the 4 out of the 5 mutants.},
 title = {Identification of Candidate Genes for Mutated Phenotype by Genome Analysis of Ion-beam-induced Rice Mutants},
 volume = {QST-M-23},
 year = {2020}
}