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Structural Studies of Overlapping Dinucleosomes in Solution
https://repo.qst.go.jp/records/78475
https://repo.qst.go.jp/records/78475504ec8d6-dc40-4e0e-bcce-e3dbab6e824b
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-08-29 | |||||
| タイトル | ||||||
| タイトル | Structural Studies of Overlapping Dinucleosomes in Solution | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Matsumoto, Atsushi
× Matsumoto, Atsushi× Sugiyama, Masaaki× Li, Zhenhai× Martel, Anne× Porcar, Lionel× Inoue, Rintaro× Kato, Daiki× Osakabe, Akihisa× Kurumizaka, Hitoshi× Kono, Hidetoshi× Matsumoto, Atsushi× Li, Zhenhai× Kono, Hidetoshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | An overlapping dinucleosome (OLDN) is a structure composed of one hexasome and one octasome and appears to be formed through nucleosome collision promoted by nucleosome remodeling factor(s). In the present study, the solution structure of the OLDN was investigated through integration of small-angle X-ray and neutron scattering (SAXS and SANS, respectively), computer modeling, and molecular dynamics simulations. Starting from the crystal structure, we generated a conformational ensemble based on normal mode analysis, and searched for the conformations that well reproduced the SAXS and SANS scattering curves. We found that inclusion of histone tails, which are not observed in the crystal structure, greatly improved model quality. The obtained structural models suggest that OLDNs adopt a variety of conformations stabilized by histone tails situated at the interface between the hexasome and octasome, simultaneously binding to both the hexasomal and octasomal DNA. In addition, our models define a possible direction for the conformational changes or dynamics, which may provide important information that furthers our understanding of the role of chromatin dynamics in gene regulation. | |||||
| 書誌情報 |
Biophysical journal 巻 118, 号 9, p. 2209-2219, 発行日 2020-01 |
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| 出版者 | ||||||
| 出版者 | Cell Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-3495 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.bpj.2019.12.010 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.biorxiv.org/content/10.1101/753327v1 | |||||
