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Synthesis and maintenance of lipid droplets are essential for mouse preimplantation embryonic development.
https://repo.qst.go.jp/records/77736
https://repo.qst.go.jp/records/7773601fbe150-c58d-4028-ae2c-35e77141ad70
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-29 | |||||
| タイトル | ||||||
| タイトル | Synthesis and maintenance of lipid droplets are essential for mouse preimplantation embryonic development. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Aizawa, Ryutaro
× Aizawa, Ryutaro× Ibayashi, Megumi× Tatsumi, Takayuki× Yamamoto, Atsushi× Kokubo, Toshiaki× Miyasaka, Naoyuki× Sato, Ken× Ikeda, Shuntaro× Minami, Naojiro× Tsukamoto, Satoshi× Ryutaro, Aizawa× Toshiaki, Kokubo× Satoshi, Tsukamoto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Lipid droplets (LDs), which are ubiquitous organelles consisting of a neutral lipid core coated with a phospholipid monolayer, play key roles in the regulation of cellular lipid metabolism. Although it is well known that mammalian oocytes and embryos contain LDs and that the amount of LDs varies among animal species, their physiological functions remain unclear. In this study, we have developed a method based on two-step centrifugation for efficient removal of almost all LDs from mouse MII oocytes (delipidation). We found that delipidated MII oocytes could be fertilized , and developed normally to the blastocyst stage even when the embryos were cultured in the absence of a fatty acid supply. LDs were newly synthesized and accumulated soon after delipidation, but chemical inhibition of long chain acyl-CoA synthetases (ACSLs) blocked this process, resulting in severe impairment of early embryonic development. Furthermore, we found that overabundance of LDs is detrimental to early embryonic development. Our findings demonstrate the importance of synthesis and maintenance of LDs, mediated in part by ACSL activity, during preimplantation embryonic development. | |||||
| 書誌情報 |
Development (Cambridge, England) 巻 146, 号 22, p. dev181925, 発行日 2019-11 |
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| 出版者 | ||||||
| 出版者 | Company Of Biologists Limited | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0950-1991 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 31772031 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1242/dev.181925 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://dev.biologists.org/content/146/22/dev181925.long | |||||
