@article{oai:repo.qst.go.jp:00077736,
 author = {Aizawa, Ryutaro and Ibayashi, Megumi and Tatsumi, Takayuki and Yamamoto, Atsushi and Kokubo, Toshiaki and Miyasaka, Naoyuki and Sato, Ken and Ikeda, Shuntaro and Minami, Naojiro and Tsukamoto, Satoshi and Ryutaro, Aizawa and Toshiaki, Kokubo and Satoshi, Tsukamoto},
 issue = {22},
 journal = {Development (Cambridge, England)},
 month = {Nov},
 note = {Lipid droplets (LDs), which are ubiquitous organelles consisting of a neutral lipid core coated with a phospholipid monolayer, play key roles in the regulation of cellular lipid metabolism. Although it is well known that mammalian oocytes and embryos contain LDs and that the amount of LDs varies among animal species, their physiological functions remain unclear. In this study, we have developed a method based on two-step centrifugation for efficient removal of almost all LDs from mouse MII oocytes (delipidation). We found that delipidated MII oocytes could be fertilized , and developed normally to the blastocyst stage even when the embryos were cultured in the absence of a fatty acid supply. LDs were newly synthesized and accumulated soon after delipidation, but chemical inhibition of long chain acyl-CoA synthetases (ACSLs) blocked this process, resulting in severe impairment of early embryonic development. Furthermore, we found that overabundance of LDs is detrimental to early embryonic development. Our findings demonstrate the importance of synthesis and maintenance of LDs, mediated in part by ACSL activity, during preimplantation embryonic development.},
 title = {Synthesis and maintenance of lipid droplets are essential for mouse preimplantation embryonic development.},
 volume = {146},
 year = {2019}
}