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Spatial distribution of DNA double-strand breaks from focused proton tracks in human cells following SPICE microbeam irradiation
https://repo.qst.go.jp/records/77501
https://repo.qst.go.jp/records/77501513f5c59-c9a7-45b1-b225-d6df276eab16
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-19 | |||||
| タイトル | ||||||
| タイトル | Spatial distribution of DNA double-strand breaks from focused proton tracks in human cells following SPICE microbeam irradiation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ohsawa, Daisuke
× Ohsawa, Daisuke× Kobayashi, Alisa× Konishi, Teruaki× Ohsawa, Daisuke× Kobayashi, Alisa× Konishi, Teruaki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Single Particle Irradiation system to Cell, SPICE, at the National Institute of Radiological Sciences(NIRS) is an advanced outstanding cell irradiation system capable of a single-proton single-cell irradiation with high throughput and without losing accuracy. It delivers a focused vertical microbeam of 3.4 MeV protons to either or both of a targeted cell nucleus and cytoplasm. Such advantages of the SPICE have motivated further investigations on the cellular response to different DNA damage complexities induced by ionizing radiation. Previously, we reported an accurate and systematic investigation of the SPICE microbeam profile using a fluorescent nuclear track detector (FNTD). In the present study, as a second step, we discuss the localized dose distribution in targeted nuclei of human lung normal WI-38 cells and its correlation with intensity and area of DNA double strand breaks (DSBs) region, visualized by immunostaining against g-H2AX. Finally, superlinear dependence of the g-H2AX spot area to the beam area was interestingly observed for a large number of protons per position, suggesting that the penumbra dose fraction in the localized dose distribution significantly contributed to the DSB induction. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 3rd QST International Symposium on Quantum Life Science | |||||
| 発表年月日 | ||||||
| 日付 | 2019-12-04 | |||||
| 日付タイプ | Issued | |||||
