@misc{oai:repo.qst.go.jp:00077501,
 author = {Ohsawa, Daisuke and Kobayashi, Alisa and Konishi, Teruaki and Ohsawa, Daisuke and Kobayashi, Alisa and Konishi, Teruaki},
 month = {Dec},
 note = {Single Particle Irradiation system to Cell, SPICE, at the National Institute of Radiological Sciences(NIRS) is an advanced outstanding cell irradiation system capable of a single-proton single-cell irradiation with high throughput and without losing accuracy. It delivers a focused vertical microbeam of 3.4 MeV protons to either or both of a targeted cell nucleus and cytoplasm. Such advantages of the SPICE have motivated further investigations on the cellular response to different DNA damage complexities induced by ionizing radiation. Previously, we reported an accurate and systematic investigation of the SPICE microbeam profile using a fluorescent nuclear track detector (FNTD). In the present study, as a second step, we discuss the localized dose distribution in targeted nuclei of human lung normal WI-38 cells and its correlation with intensity and area of DNA double strand breaks (DSBs) region, visualized by immunostaining against g-H2AX. Finally, superlinear dependence of the g-H2AX spot area to the beam area was interestingly observed for a large
number of protons per position, suggesting that the penumbra dose fraction in the localized dose distribution significantly contributed to the DSB induction., 3rd QST International Symposium on Quantum Life Science},
 title = {Spatial distribution of DNA double-strand breaks from focused proton tracks in human cells following SPICE microbeam irradiation},
 year = {2019}
}