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Repair susceptibility of hydrated EGFP-expressing plasmid DNA transfected into human cells after exposure to X-rays
https://repo.qst.go.jp/records/77419
https://repo.qst.go.jp/records/774195e62ef54-d238-44ee-b128-d7df4fcf03bb
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-11-13 | |||||
| タイトル | ||||||
| タイトル | Repair susceptibility of hydrated EGFP-expressing plasmid DNA transfected into human cells after exposure to X-rays | |||||
| タイトル | ||||||
| タイトル | ヒト細胞中に移入されたX 線照射水和EGFP 発現プラスミド DNA の修復感受性 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirasaki, Keishiro
× Hirasaki, Keishiro× Obata, Yui× Yokoya, Akinari× Hirasaki, Keishiro× Obata, Yui× Yokoya, Akinari |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Clustered DNA damage is defined as two or more lesions (base lesions, single strand breaks or abasic (AP) sites ) localized within one or two helical turn(s). Although repair susceptibility of a synthesized base lesion (or AP) cluster in oligonucleotides have been investigated, those induced actually by irradiation is not clarified yet. To elucidate the repair susceptibility of radiation- induced clustered damage, in the present study, fully hydrated DNA films containing 35 water molecules per nucleotide, as well as DNA solutions (1×TE, 1μg/ μl) were used as samples. In the hydrated DNA films, diffusible water radicals are hardly generated by ionizing radiation. Thus, the major process of radiation action is direct ionization or impact of secondary electrons to DNA. The EGFP-expressing plasmids were exposed to X-rays with a 1/e dose for each sample, and then transfected into non-irradiated human cells (MCF-7). Those cells were observed for 48h with fluorescence microscope. DNA repair efficiencies were obtained as the number of fluorescence expression cells. The efficiency for the hydrated DNA film was significantly lower than that for the solution sample. These results indicate that DNA damage induced in the hydrated DNA would be clustered lesions and difficult to be repaired. |
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| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 日本放射線影響学会第62回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-11-15 | |||||
| 日付タイプ | Issued | |||||
