{"created":"2023-05-15T14:57:03.175604+00:00","id":77419,"links":{},"metadata":{"_buckets":{"deposit":"2d45f111-6733-42d9-bdf6-9b0b4b0658fc"},"_deposit":{"created_by":1,"id":"77419","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"77419"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00077419","sets":["10:28"]},"author_link":["859964","859960","859961","859962","859963","859959"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-11-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Clustered DNA damage is defined as two or more\nlesions (base lesions, single strand breaks or abasic\n(AP) sites ) localized within one or two helical turn(s).\nAlthough repair susceptibility of a synthesized base\nlesion (or AP) cluster in oligonucleotides have been\ninvestigated, those induced actually by irradiation is not\nclarified yet. To elucidate the repair susceptibility of radiation-\ninduced clustered damage, in the present study,\nfully hydrated DNA films containing 35 water molecules\nper nucleotide, as well as DNA solutions (1×TE, 1μg/\nμl) were used as samples. In the hydrated DNA films,\ndiffusible water radicals are hardly generated by ionizing\nradiation. Thus, the major process of radiation action\nis direct ionization or impact of secondary electrons to\nDNA. The EGFP-expressing plasmids were exposed\nto X-rays with a 1/e dose for each sample, and then\ntransfected into non-irradiated human cells (MCF-7).\nThose cells were observed for 48h with fluorescence microscope.\nDNA repair efficiencies were obtained as the\nnumber of fluorescence expression cells. The efficiency\nfor the hydrated DNA film was significantly lower than\nthat for the solution sample. These results indicate that\nDNA damage induced in the hydrated DNA would be\nclustered lesions and difficult to be repaired.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"日本放射線影響学会第62回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirasaki, Keishiro"}],"nameIdentifiers":[{"nameIdentifier":"859959","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Obata, Yui"}],"nameIdentifiers":[{"nameIdentifier":"859960","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokoya, Akinari"}],"nameIdentifiers":[{"nameIdentifier":"859961","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirasaki, Keishiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"859962","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Obata, Yui","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"859963","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokoya, Akinari","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"859964","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Repair susceptibility of hydrated EGFP-expressing plasmid DNA transfected into human cells after exposure to X-rays","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Repair susceptibility of hydrated EGFP-expressing plasmid DNA transfected into human cells after exposure to X-rays"},{"subitem_title":"ヒト細胞中に移入されたX 線照射水和EGFP 発現プラスミド DNA の修復感受性","subitem_title_language":"en"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-11-13"},"publish_date":"2019-11-13","publish_status":"0","recid":"77419","relation_version_is_last":true,"title":["Repair susceptibility of hydrated EGFP-expressing plasmid DNA transfected into human cells after exposure to X-rays"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T22:12:17.845041+00:00"}