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Study of radiation-induced clustered DNA damage by fluorescence anisotropy measurement based on Förster resonance energy transfer
https://repo.qst.go.jp/records/76640
https://repo.qst.go.jp/records/766409736294b-de85-42d6-9a87-55cb9fcf9aad
| アイテムタイプ | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-09-03 | |||||
| タイトル | ||||||
| タイトル | Study of radiation-induced clustered DNA damage by fluorescence anisotropy measurement based on Förster resonance energy transfer | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference output | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Akamatsu, Ken
× Akamatsu, Ken× Shikazono, Naoya× Akamatsu, Ken× Shikazono, Naoya |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | It is well-known that DNA lesions induced by ionizing radiation and chemicals can cause mutation and carcinogenesis. In particular, “clustered damage" site, that is a DNA region with multiple lesions within a few helical turns, is believed to hardly be repaired. This type of damage is considered to be induced around high-LET radiation tracks and at a track-end of secondary electron. However, chemical and spatial details of them are not known. We have developed a methodology for estimating localization of the lesions using fluorescence anisotropy. The fluorescence anisotropy generally decreases with increasing Förster resonance energy transfer (FRET) efficiency. We found that experimentally-obtained anisotropy for the heat-treated DNA correspond to theoretical ones calculated on the basis of exponential distribution. Now we are applying the methodology based on fluorescence anisotropy to liner-formed pUC19 irradiated with radiation such as Co-60 gamma-rays, helium, carbon, and Fe ion beams. The results and the prospective will be discussed. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ICRR(International Congress on Radiation Research) 2019 | |||||
| 発表年月日 | ||||||
| 日付 | 2019-08-26 | |||||
| 日付タイプ | Issued | |||||
