WEKO3
アイテム
Non-homologous end joining repair pathway determines cell radiosensitivity in helium and carbon ion beams
https://repo.qst.go.jp/records/76306
https://repo.qst.go.jp/records/76306d75ef9bd-6574-46d8-8746-7a1a58ebc8e3
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2019-03-08 | |||||
| タイトル | ||||||
| タイトル | Non-homologous end joining repair pathway determines cell radiosensitivity in helium and carbon ion beams | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
B. Flint, David
× B. Flint, David× J. Bright, Scott× H. McFadden, Conor× Chakraborty, Sharmistha× S. Yoon, David× F. Shaitelman, Simona× Kodaira, Satoshi× Konishi, Teruaki× O. Sawakuchi, Gabriel× Satoshi, Kodaira× Teruaki, Konishi |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose: To highlight the importance of non-homologous end-joining (NHEJ) repair in governing a cell’s radiosensitivity to helium and carbon ion beams of varying linear energy transfer (LET). Method and Materials: We selected a pair of isogenic cancer cell lines with differing proficiency in non-homologous end joining (NHEJ) repair: M059K (NHEJ-proficient) and M059J (NHEJ-deficient) and as additional reference cell lines, we selected the H1299 non-small cell lung cancer and H460 large cell lung cancer cell lines. These cells were exposed to unmodulated helium and carbon ion beams of nominal energy 150 MeV/u (helium) and 290 MeV/u (carbon) and energy degraders were used to achieve LETs ranging from 2.2 to 60 keV/µm. We performed clonogenic assays to calculate the relative biological effectiveness (RBE) of each cell line in these conditions, relative to their survival in 6 MV x-rays, using the dose for 10% survival as the biological endpoint. To support the survival data, we performed immunohistochemical staining of DNA double strand break (DSB) markers (including γH2AX and 53BP1) 30 min and 24 h post irradiation to identify persistent radiation induced foci. Results: The RBE of the NHEJ-deficient M059J cell line showed significantly less LET dependence than the reference cell lines (increasing only to 1.37± 0.07 at 60 keV/μm for M059J versus 1.80 ± 0.10, 1.79 ± 0.04 and 1.98 ± 0.05 for M059K, H460 and H1299, respectively), despite M059J being more radiosensitive to all LETs. Preliminary analysis of foci data for proton-exposed cells indicate that the M059J cells have more persistent DSB repair foci 24 h post-irradiation, consistent with their deficiency in DNA DSB repair. Conclusions: Our results indicate that proficiency status in NHEJ is extremely important in determining how cell radiosensitivity varies with He- and C-ion LET. |
|||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The 61st AAPM Annual Meeting and Exhibition | |||||
| 発表年月日 | ||||||
| 日付 | 2019-07-15 | |||||
| 日付タイプ | Issued | |||||
