{"created":"2023-05-15T14:56:11.117732+00:00","id":76306,"links":{},"metadata":{"_buckets":{"deposit":"595e85ba-3ef3-40fa-b62c-fbe478b2837b"},"_deposit":{"created_by":1,"id":"76306","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"76306"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00076306","sets":["10:28"]},"author_link":["1070934","1070941","1070937","1070932","1070940","1070936","1070939","1070935","1070938","1070933","1070942"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2019-07-15","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: To highlight the importance of non-homologous end-joining (NHEJ) repair in governing a cell’s radiosensitivity to helium and carbon ion beams of varying linear energy transfer (LET).\nMethod and Materials: We selected a pair of isogenic cancer cell lines with differing proficiency in non-homologous end joining (NHEJ) repair: M059K (NHEJ-proficient) and M059J (NHEJ-deficient) and as additional reference cell lines, we selected the H1299 non-small cell lung cancer and H460 large cell lung cancer cell lines. These cells were exposed to unmodulated helium and carbon ion beams of nominal energy 150 MeV/u (helium) and 290 MeV/u (carbon) and energy degraders were used to achieve LETs ranging from 2.2 to 60 keV/µm. We performed clonogenic assays to calculate the relative biological effectiveness (RBE) of each cell line in these conditions, relative to their survival in 6 MV x-rays, using the dose for 10% survival as the biological endpoint. To support the survival data, we performed immunohistochemical staining of DNA double strand break (DSB) markers (including γH2AX and 53BP1) 30 min and 24 h post irradiation to identify persistent radiation induced foci.\nResults: The RBE of the NHEJ-deficient M059J cell line showed significantly less LET dependence than the reference cell lines (increasing only to 1.37± 0.07 at 60 keV/μm for M059J versus 1.80 ± 0.10, 1.79 ± 0.04 and 1.98 ± 0.05 for M059K, H460 and H1299, respectively), despite M059J being more radiosensitive to all LETs. Preliminary analysis of foci data for proton-exposed cells indicate that the M059J cells have more persistent DSB repair foci 24 h post-irradiation, consistent with their deficiency in DNA DSB repair. \n Conclusions: Our results indicate that proficiency status in NHEJ is extremely important in determining how cell radiosensitivity varies with He- and C-ion LET. \n","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要(会議名, 開催地, 会期, 主催者等)","attribute_value_mlt":[{"subitem_description":"The 61st AAPM Annual Meeting and Exhibition","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"B. Flint, David"}],"nameIdentifiers":[{"nameIdentifier":"1070932","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"J. Bright, Scott"}],"nameIdentifiers":[{"nameIdentifier":"1070933","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"H. McFadden, Conor"}],"nameIdentifiers":[{"nameIdentifier":"1070934","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chakraborty, Sharmistha"}],"nameIdentifiers":[{"nameIdentifier":"1070935","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"S. Yoon, David"}],"nameIdentifiers":[{"nameIdentifier":"1070936","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"F. Shaitelman, Simona"}],"nameIdentifiers":[{"nameIdentifier":"1070937","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kodaira, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"1070938","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Konishi, Teruaki"}],"nameIdentifiers":[{"nameIdentifier":"1070939","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"O. Sawakuchi, Gabriel"}],"nameIdentifiers":[{"nameIdentifier":"1070940","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Satoshi, Kodaira","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1070941","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Teruaki, Konishi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1070942","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Non-homologous end joining repair pathway determines cell radiosensitivity in helium and carbon ion beams","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Non-homologous end joining repair pathway determines cell radiosensitivity in helium and carbon ion beams"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-08"},"publish_date":"2019-03-08","publish_status":"0","recid":"76306","relation_version_is_last":true,"title":["Non-homologous end joining repair pathway determines cell radiosensitivity in helium and carbon ion beams"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T16:44:47.929751+00:00"}