{"_buckets": {"deposit": "ffecaafc-3a52-41cd-8fab-3b95bc822418"}, "_deposit": {"created_by": 1, "id": "73849", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "73849"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00073849", "sets": ["1"]}, "author_link": ["872911", "872907", "872914", "872909", "872912", "872916", "872915", "872918", "872910", "872908", "872905", "872913", "872906", "872917"], "item_8_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{"bibliographic_title": "Theranostics"}]}]}, "item_8_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Targeted α-particle therapy is a promising option for patients with malignant pheochromocytoma. Recent observations regarding meta-211At-astato-benzylguanidine (211At-MABG) in a pheochromocytoma mouse model showed a strong anti-tumor effect, though the molecular mechanism remains elusive. Here, we present the first comprehensive RNA-sequencing (RNA-seq) data for pheochromocytoma cells based on in vitro 211At-MABG administration experiments. Key genes and pathways in the tumor α-particle radiation response are also examined to obtain potential response biomarkers.\nMethods: We evaluated genome-wide transcriptional alterations in the rat pheochromocytoma cell line PC12 at 3, 6, and 12 h after 211At-MABG treatment; a control experiment using 60Co γ-ray irradiation was carried out to highlight 211At-MABG-specific gene expression. For comparisons, 10% and 80% iso-survival doses (0.8 and 0.1 kBq/mL for 211At-MABG and 10 and 1 Gy for 60Co γ-rays) were used.\nResults: Enrichment analysis of differentially expressed genes (DEGs) and analysis of the gene expression profiles of cell cycle checkpoints revealed similar modes of cell death via the p53-p21 signaling pathway after 211At-MABG treatment and γ-ray irradiation. The top list of ranked DEGs demonstrated the expression of key genes on the decrease in the survival following 211At-MABG exposure, and four potential genes (Mien1, Otub1, Vdac1 and Vegfa genes) of 211At-MABG therapy. Western blot analysis indicated increased expression of TSPO in 211At-MABG-treated cells, suggesting its potential as a PET imaging probe.\nConclusion: Comprehensive RNA-seq revealed contrasting cellular responses to γ-ray and α-particle therapy, leading to the identification of four potential candidate genes that may serve as molecular imaging and 211At-MABG therapy targets.", "subitem_description_type": "Abstract"}]}, "item_8_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "IVYSPRING"}]}, "item_8_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "10.7150/thno.30353", "subitem_relation_type_select": "DOI"}}]}, "item_8_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1838-7640", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "大島, 康宏"}], "nameIdentifiers": [{"nameIdentifier": "872905", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "河野, 暢明"}], "nameIdentifiers": [{"nameIdentifier": "872906", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "横田, 裕一郎"}], "nameIdentifiers": [{"nameIdentifier": "872907", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "渡辺, 茂樹"}], "nameIdentifiers": [{"nameIdentifier": "872908", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "佐々木, 一郎"}], "nameIdentifiers": [{"nameIdentifier": "872909", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "石岡, 典子"}], "nameIdentifiers": [{"nameIdentifier": "872910", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "坂下, 哲哉"}], "nameIdentifiers": [{"nameIdentifier": "872911", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "荒川, 和晴"}], "nameIdentifiers": [{"nameIdentifier": "872912", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oshima, Yasuhiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "872913", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yokota, Yuuichiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "872914", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Shigeki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "872915", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sasaki, Ichiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "872916", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishioka, Noriko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "872917", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakashita, Tetsuya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "872918", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Anti-tumor effects and potential therapeutic response biomarkers in alpha-emitting meta-At-211-astato-benzylguanidine therapy for malignant pheochromocytoma explored by RNA-sequencing", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Anti-tumor effects and potential therapeutic response biomarkers in alpha-emitting meta-At-211-astato-benzylguanidine therapy for malignant pheochromocytoma explored by RNA-sequencing"}]}, "item_type_id": "8", "owner": "1", "path": ["1"], "permalink_uri": "https://repo.qst.go.jp/records/73849", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-02-27"}, "publish_date": "2019-02-27", "publish_status": "0", "recid": "73849", "relation": {}, "relation_version_is_last": true, "title": ["Anti-tumor effects and potential therapeutic response biomarkers in alpha-emitting meta-At-211-astato-benzylguanidine therapy for malignant pheochromocytoma explored by RNA-sequencing"], "weko_shared_id": -1}