{"created":"2023-05-15T14:54:20.636355+00:00","id":73849,"links":{},"metadata":{"_buckets":{"deposit":"ffecaafc-3a52-41cd-8fab-3b95bc822418"},"_deposit":{"created_by":1,"id":"73849","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"73849"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00073849","sets":["1"]},"author_link":["872911","872907","872914","872909","872912","872916","872915","872918","872910","872908","872905","872913","872906","872917"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"Theranostics"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Targeted α-particle therapy is a promising option for patients with malignant pheochromocytoma. Recent observations regarding meta-211At-astato-benzylguanidine (211At-MABG) in a pheochromocytoma mouse model showed a strong anti-tumor effect, though the molecular mechanism remains elusive. Here, we present the first comprehensive RNA-sequencing (RNA-seq) data for pheochromocytoma cells based on in vitro 211At-MABG administration experiments. Key genes and pathways in the tumor α-particle radiation response are also examined to obtain potential response biomarkers.\nMethods: We evaluated genome-wide transcriptional alterations in the rat pheochromocytoma cell line PC12 at 3, 6, and 12 h after 211At-MABG treatment; a control experiment using 60Co γ-ray irradiation was carried out to highlight 211At-MABG-specific gene expression. For comparisons, 10% and 80% iso-survival doses (0.8 and 0.1 kBq/mL for 211At-MABG and 10 and 1 Gy for 60Co γ-rays) were used.\nResults: Enrichment analysis of differentially expressed genes (DEGs) and analysis of the gene expression profiles of cell cycle checkpoints revealed similar modes of cell death via the p53-p21 signaling pathway after 211At-MABG treatment and γ-ray irradiation. The top list of ranked DEGs demonstrated the expression of key genes on the decrease in the survival following 211At-MABG exposure, and four potential genes (Mien1, Otub1, Vdac1 and Vegfa genes) of 211At-MABG therapy. Western blot analysis indicated increased expression of TSPO in 211At-MABG-treated cells, suggesting its potential as a PET imaging probe.\nConclusion: Comprehensive RNA-seq revealed contrasting cellular responses to γ-ray and α-particle therapy, leading to the identification of four potential candidate genes that may serve as molecular imaging and 211At-MABG therapy targets.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"IVYSPRING"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.7150/thno.30353","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1838-7640","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大島, 康宏"}],"nameIdentifiers":[{"nameIdentifier":"872905","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"河野, 暢明"}],"nameIdentifiers":[{"nameIdentifier":"872906","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"横田, 裕一郎"}],"nameIdentifiers":[{"nameIdentifier":"872907","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"渡辺, 茂樹"}],"nameIdentifiers":[{"nameIdentifier":"872908","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木, 一郎"}],"nameIdentifiers":[{"nameIdentifier":"872909","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石岡, 典子"}],"nameIdentifiers":[{"nameIdentifier":"872910","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂下, 哲哉"}],"nameIdentifiers":[{"nameIdentifier":"872911","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荒川, 和晴"}],"nameIdentifiers":[{"nameIdentifier":"872912","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oshima, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yokota, Yuuichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872914","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Shigeki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872915","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasaki, Ichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872916","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishioka, Noriko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872917","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakashita, Tetsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"872918","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Anti-tumor effects and potential therapeutic response biomarkers in alpha-emitting meta-At-211-astato-benzylguanidine therapy for malignant pheochromocytoma explored by RNA-sequencing","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Anti-tumor effects and potential therapeutic response biomarkers in alpha-emitting meta-At-211-astato-benzylguanidine therapy for malignant pheochromocytoma explored by RNA-sequencing"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-27"},"publish_date":"2019-02-27","publish_status":"0","recid":"73849","relation_version_is_last":true,"title":["Anti-tumor effects and potential therapeutic response biomarkers in alpha-emitting meta-At-211-astato-benzylguanidine therapy for malignant pheochromocytoma explored by RNA-sequencing"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T21:50:14.949707+00:00"}