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Designing an artificial transcription factor with a small molecular weight based on engrailed homeodomain
https://repo.qst.go.jp/records/72940
https://repo.qst.go.jp/records/729405a854c17-627f-4ced-a97a-0860500a7288
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-09-18 | |||||
| タイトル | ||||||
| タイトル | Designing an artificial transcription factor with a small molecular weight based on engrailed homeodomain | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
T., Sunami
× T., Sunami× H., Kono× 角南 智子× 河野 秀俊 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Widely used genome editing enzymes such as TALEN and CRISPR have a high molecular weight. To develop a novel genome editing enzyme that works in a condensed environment, an enzymes with a smaller molecular weight is desired. We use the engralied homeodomain protein as such. The wild type recognizes AT-rich 6bps. To create a protein that recognize longer DNA sequence, we connected two homeodomains with a linker of different lengths and characterized them by EMSA and B1H assay. They showed a good activity for our target sequence, however, they also bound to undesired sequences. To avoid the undesired binding, we created some mutants and confirmed their better specificity. We are now solving the crystal structure of the complex to see if the binding form is as expected. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 第56回日本生物物理学会年会 | |||||
| 発表年月日 | ||||||
| 日付 | 2018-09-17 | |||||
| 日付タイプ | Issued | |||||
