@misc{oai:repo.qst.go.jp:00072940,
 author = {T., Sunami and H., Kono and 角南 智子 and 河野 秀俊},
 month = {Sep},
 note = {Widely used genome editing enzymes such as TALEN and CRISPR have a high molecular weight. To develop a novel genome editing enzyme　that works in a condensed environment, an enzymes with a smaller molecular weight is desired. We use the engralied homeodomain protein as such. The wild type recognizes AT-rich 6bps. To create a protein that recognize longer DNA sequence, we connected two homeodomains with a linker of different lengths and characterized them by EMSA and B1H assay. They showed a good activity for our target sequence, however, they also bound to undesired sequences. To avoid the undesired binding, we created some mutants and confirmed their better specificity. We are now solving the crystal structure of the complex to see if the binding form is as expected., 第56回日本生物物理学会年会},
 title = {Designing an artificial transcription factor with a small molecular weight based on engrailed homeodomain},
 year = {2018}
}