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Study on functions of the DNA damage response factors in the cellular response to heavy ion beams
https://repo.qst.go.jp/records/72287
https://repo.qst.go.jp/records/72287c770819f-ddbe-4829-a7f7-dddb8cdbf1d4
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-04-20 | |||||
| タイトル | ||||||
| タイトル | Study on functions of the DNA damage response factors in the cellular response to heavy ion beams | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Qiang, LIU
× Qiang, LIU× Yi, XIE× Liqing, DU× Yan, WANG× Chang, XU× Mengzheng, GUO× 勝部, 孝則× 二宮, 康晴× 王, 冰× 勝部 孝則× 二宮 康晴× 王 冰 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A synthetic polypeptide ANTP- SmacN7 is a possible radiosensitizing agent derived from N terminal 7 amino acid sequence of Smac protein, which antagonizes a caspase inhibitor XIAP. To investigate the underlying mechanisms behind radiosensitization, we analyzed cellular responses of two non-small cell lung carcinomas (NSCLC) cell lines, H460 and A549, to low- and high-LET ionizing radiation (IR) and ANTP-smacN7. Western analysis revealed that expression of XIAP was higher in A549 than in H460. Sensitivities of these cells to iron (LET=200 kev/μm), carbon (LET=70 kev/μm) and photon (200 kvp X-rays) beams were determined by clonogenic assay. A549 cells were 1.6-2.0-fold more resistant to these IRs than H460, based on the doses required to reduce the cell survival to 50% (LD50). Flow cytometry assay.0s revealed that radio-induced cellular apoptosis emerged at 6 h and reached a peak level at 24 after IR at LD50. Notably, administration of ANTP-SmacN7 reduced the cell survival of A549 and H460 cell by 56% and 30%, respectively, after the iron irradiation at each LD50. These results suggested that ANTP-SmacN7 promotes apoptotic response to IR by antagonizing inhibitory effect of XIAP on caspase and thus being effective for cells particularly with high XIAP expression. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | H28年度HIMAC共同利用研究成果発表会 | |||||
| 発表年月日 | ||||||
| 日付 | 2017-04-17 | |||||
| 日付タイプ | Issued | |||||
