{"created":"2023-05-15T14:52:57.930501+00:00","id":72287,"links":{},"metadata":{"_buckets":{"deposit":"5a66aee8-d1d6-4c64-ad3f-ed9418e00a87"},"_deposit":{"created_by":1,"id":"72287","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"72287"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00072287","sets":["10:28"]},"author_link":["711990","711987","711989","711993","711983","711986","711985","711984","711991","711988","711994","711992"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2017-04-17","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A synthetic polypeptide ANTP- SmacN7 is a possible radiosensitizing agent derived from N terminal 7 amino acid sequence of Smac protein, which antagonizes a caspase inhibitor XIAP. To investigate the underlying mechanisms behind radiosensitization, we analyzed cellular responses of two non-small cell lung carcinomas (NSCLC) cell lines, H460 and A549, to low- and high-LET ionizing radiation (IR) and ANTP-smacN7. Western analysis revealed that expression of XIAP was higher in A549 than in H460. Sensitivities of these cells to iron (LET=200 kev/μm), carbon (LET=70 kev/μm) and photon (200 kvp X-rays) beams were determined by clonogenic assay. A549 cells were 1.6-2.0-fold more resistant to these IRs than H460, based on the doses required to reduce the cell survival to 50% (LD50). Flow cytometry assay.0s revealed that radio-induced cellular apoptosis emerged at 6 h and reached a peak level at 24 after IR at LD50. Notably, administration of ANTP-SmacN7 reduced the cell survival of A549 and H460 cell by 56% and 30%, respectively, after the iron irradiation at each LD50. These results suggested that ANTP-SmacN7 promotes apoptotic response to IR by antagonizing inhibitory effect of XIAP on caspase and thus being effective for cells particularly with high XIAP expression.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"H28年度HIMAC共同利用研究成果発表会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Qiang, LIU"}],"nameIdentifiers":[{"nameIdentifier":"711983","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yi, XIE"}],"nameIdentifiers":[{"nameIdentifier":"711984","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Liqing, DU"}],"nameIdentifiers":[{"nameIdentifier":"711985","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yan, WANG"}],"nameIdentifiers":[{"nameIdentifier":"711986","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Chang, XU"}],"nameIdentifiers":[{"nameIdentifier":"711987","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mengzheng, GUO"}],"nameIdentifiers":[{"nameIdentifier":"711988","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"勝部, 孝則"}],"nameIdentifiers":[{"nameIdentifier":"711989","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"二宮, 康晴"}],"nameIdentifiers":[{"nameIdentifier":"711990","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王, 冰"}],"nameIdentifiers":[{"nameIdentifier":"711991","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"勝部 孝則","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"711992","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"二宮 康晴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"711993","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 冰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"711994","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"Study on functions of the DNA damage response factors in the cellular response to heavy ion beams","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Study on functions of the DNA damage response factors in the cellular response to heavy ion beams"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-04-20"},"publish_date":"2017-04-20","publish_status":"0","recid":"72287","relation_version_is_last":true,"title":["Study on functions of the DNA damage response factors in the cellular response to heavy ion beams"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:41:37.783966+00:00"}