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High Radiosensitivity of Bone Marrow Cells in GFP Transgenic Mice
https://repo.qst.go.jp/records/71778
https://repo.qst.go.jp/records/71778733d3f8f-262d-4565-b573-f6d61283ed4c
| Item type | 会議発表用資料 / Presentation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-08-06 | |||||
| タイトル | ||||||
| タイトル | High Radiosensitivity of Bone Marrow Cells in GFP Transgenic Mice | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
| 資源タイプ | conference object | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Ninomiya, Yasuharu
× Ninomiya, Yasuharu× Tanaka, Kaoru× Nenoi, Mitsuru× nenoi, Mitsuru× Wang, Bing× 二宮 康晴× 田中 薫× 根井 充× 王 冰 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Transgenic mice ubiquitously expressing green fluorescent protein (GFP) are frequently used in radiobiology for various purposes. But, equivalence of the GFP mice with their wild-type counterparts in regard of radiosensitivity has not been confirmed yet. In this study, we analyzed radiosensitivity of thymus, spleen and bone marrow, using GFP transgenic mice (GFP-BL/6N) and their wild-type counterparts (BL/6N). As a result, the forward and side scatter plot in flowcytometry analysis showed an elevated cell death in bone marrow of GFP-BL/6N compared to that in BL/6N after X-irradiation with 4 Gy. When radiosensitivity of each type of cells in bone marrow was analyzed by using a gate of cell sorter for myeloid and lymphoid, the number of myeloid cells of GFP-BL/6N was found to decrease more extensively than that of BL/6N 3 hr after X-irradiation with 4 Gy. TUNEL assay revealed an elevated apoptosis of myeloid cells of GFP-BL/6N compared to that of BL/6N 3 hr after X-irradiation with 4 Gy. In addition, expression of bax gene in bone marrow cells was found to be increased more extensively in GFP-BL/6N than in BL/6N after X-irradiation with 4 Gy. These results indicated bone marrow cells of GFP-BL/6N are radiosensitive to high dose of X-irradiation than that of BL/6N. | |||||
| 会議概要（会議名, 開催地, 会期, 主催者等） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ICRR2015/日本放射線影響学会第58回大会 | |||||
| 発表年月日 | ||||||
| 日付 | 2015-05-27 | |||||
| 日付タイプ | Issued | |||||
