WEKO3
アイテム
{"_buckets": {"deposit": "59071726-3785-470c-9103-f83c6349255c"}, "_deposit": {"created_by": 1, "id": "71778", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "71778"}, "status": "published"}, "_oai": {"id": "oai:repo.qst.go.jp:00071778", "sets": ["28"]}, "author_link": ["706495", "706492", "706493", "706490", "706487", "706489", "706488", "706491", "706494"], "item_10005_date_7": {"attribute_name": "発表年月日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2015-05-27", "subitem_date_issued_type": "Issued"}]}, "item_10005_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Transgenic mice ubiquitously expressing green fluorescent protein (GFP) are frequently used in radiobiology for various purposes. But, equivalence of the GFP mice with their wild-type counterparts in regard of radiosensitivity has not been confirmed yet. In this study, we analyzed radiosensitivity of thymus, spleen and bone marrow, using GFP transgenic mice (GFP-BL/6N) and their wild-type counterparts (BL/6N). As a result, the forward and side scatter plot in flowcytometry analysis showed an elevated cell death in bone marrow of GFP-BL/6N compared to that in BL/6N after X-irradiation with 4 Gy. When radiosensitivity of each type of cells in bone marrow was analyzed by using a gate of cell sorter for myeloid and lymphoid, the number of myeloid cells of GFP-BL/6N was found to decrease more extensively than that of BL/6N 3 hr after X-irradiation with 4 Gy. TUNEL assay revealed an elevated apoptosis of myeloid cells of GFP-BL/6N compared to that of BL/6N 3 hr after X-irradiation with 4 Gy. In addition, expression of bax gene in bone marrow cells was found to be increased more extensively in GFP-BL/6N than in BL/6N after X-irradiation with 4 Gy. These results indicated bone marrow cells of GFP-BL/6N are radiosensitive to high dose of X-irradiation than that of BL/6N.", "subitem_description_type": "Abstract"}]}, "item_10005_description_6": {"attribute_name": "会議概要(会議名, 開催地, 会期, 主催者等)", "attribute_value_mlt": [{"subitem_description": "ICRR2015/日本放射線影響学会第58回大会", "subitem_description_type": "Other"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ninomiya, Yasuharu"}], "nameIdentifiers": [{"nameIdentifier": "706487", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Kaoru"}], "nameIdentifiers": [{"nameIdentifier": "706488", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nenoi, Mitsuru"}], "nameIdentifiers": [{"nameIdentifier": "706489", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "nenoi, Mitsuru"}], "nameIdentifiers": [{"nameIdentifier": "706490", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wang, Bing"}], "nameIdentifiers": [{"nameIdentifier": "706491", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "二宮 康晴", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "706492", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "田中 薫", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "706493", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "根井 充", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "706494", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "王 冰", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "706495", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "conference object", "resourceuri": "http://purl.org/coar/resource_type/c_c94f"}]}, "item_title": "High Radiosensitivity of Bone Marrow Cells in GFP Transgenic Mice", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "High Radiosensitivity of Bone Marrow Cells in GFP Transgenic Mice"}]}, "item_type_id": "10005", "owner": "1", "path": ["28"], "permalink_uri": "https://repo.qst.go.jp/records/71778", "pubdate": {"attribute_name": "公開日", "attribute_value": "2015-08-06"}, "publish_date": "2015-08-06", "publish_status": "0", "recid": "71778", "relation": {}, "relation_version_is_last": true, "title": ["High Radiosensitivity of Bone Marrow Cells in GFP Transgenic Mice"], "weko_shared_id": -1}
High Radiosensitivity of Bone Marrow Cells in GFP Transgenic Mice
https://repo.qst.go.jp/records/71778
https://repo.qst.go.jp/records/71778733d3f8f-262d-4565-b573-f6d61283ed4c
Item type | 会議発表用資料 / Presentation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2015-08-06 | |||||
タイトル | ||||||
タイトル | High Radiosensitivity of Bone Marrow Cells in GFP Transgenic Mice | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_c94f | |||||
資源タイプ | conference object | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Ninomiya, Yasuharu
× Ninomiya, Yasuharu× Tanaka, Kaoru× Nenoi, Mitsuru× nenoi, Mitsuru× Wang, Bing× 二宮 康晴× 田中 薫× 根井 充× 王 冰 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Transgenic mice ubiquitously expressing green fluorescent protein (GFP) are frequently used in radiobiology for various purposes. But, equivalence of the GFP mice with their wild-type counterparts in regard of radiosensitivity has not been confirmed yet. In this study, we analyzed radiosensitivity of thymus, spleen and bone marrow, using GFP transgenic mice (GFP-BL/6N) and their wild-type counterparts (BL/6N). As a result, the forward and side scatter plot in flowcytometry analysis showed an elevated cell death in bone marrow of GFP-BL/6N compared to that in BL/6N after X-irradiation with 4 Gy. When radiosensitivity of each type of cells in bone marrow was analyzed by using a gate of cell sorter for myeloid and lymphoid, the number of myeloid cells of GFP-BL/6N was found to decrease more extensively than that of BL/6N 3 hr after X-irradiation with 4 Gy. TUNEL assay revealed an elevated apoptosis of myeloid cells of GFP-BL/6N compared to that of BL/6N 3 hr after X-irradiation with 4 Gy. In addition, expression of bax gene in bone marrow cells was found to be increased more extensively in GFP-BL/6N than in BL/6N after X-irradiation with 4 Gy. These results indicated bone marrow cells of GFP-BL/6N are radiosensitive to high dose of X-irradiation than that of BL/6N. | |||||
会議概要(会議名, 開催地, 会期, 主催者等) | ||||||
内容記述タイプ | Other | |||||
内容記述 | ICRR2015/日本放射線影響学会第58回大会 | |||||
発表年月日 | ||||||
日付 | 2015-05-27 | |||||
日付タイプ | Issued |