{"created":"2023-05-15T14:52:34.408965+00:00","id":71778,"links":{},"metadata":{"_buckets":{"deposit":"59071726-3785-470c-9103-f83c6349255c"},"_deposit":{"created_by":1,"id":"71778","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"71778"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00071778","sets":["10:28"]},"author_link":["706495","706492","706493","706490","706487","706489","706488","706491","706494"],"item_10005_date_7":{"attribute_name":"発表年月日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-05-27","subitem_date_issued_type":"Issued"}]},"item_10005_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transgenic mice ubiquitously expressing green fluorescent protein (GFP) are frequently used in radiobiology for various purposes. But, equivalence of the GFP mice with their wild-type counterparts in regard of radiosensitivity has not been confirmed yet. In this study, we analyzed radiosensitivity of thymus, spleen and bone marrow, using GFP transgenic mice (GFP-BL/6N) and their wild-type counterparts (BL/6N). As a result, the forward and side scatter plot in flowcytometry analysis showed an elevated cell death in bone marrow of GFP-BL/6N compared to that in BL/6N after X-irradiation with 4 Gy. When radiosensitivity of each type of cells in bone marrow was analyzed by using a gate of cell sorter for myeloid and lymphoid, the number of myeloid cells of GFP-BL/6N was found to decrease more extensively than that of BL/6N 3 hr after X-irradiation with 4 Gy. TUNEL assay revealed an elevated apoptosis of myeloid cells of GFP-BL/6N compared to that of BL/6N 3 hr after X-irradiation with 4 Gy. In addition, expression of bax gene in bone marrow cells was found to be increased more extensively in GFP-BL/6N than in BL/6N after X-irradiation with 4 Gy. These results indicated bone marrow cells of GFP-BL/6N are radiosensitive to high dose of X-irradiation than that of BL/6N.","subitem_description_type":"Abstract"}]},"item_10005_description_6":{"attribute_name":"会議概要（会議名, 開催地, 会期, 主催者等）","attribute_value_mlt":[{"subitem_description":"ICRR2015/日本放射線影響学会第58回大会","subitem_description_type":"Other"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ninomiya, Yasuharu"}],"nameIdentifiers":[{"nameIdentifier":"706487","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Kaoru"}],"nameIdentifiers":[{"nameIdentifier":"706488","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"706489","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"nenoi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"706490","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wang, Bing"}],"nameIdentifiers":[{"nameIdentifier":"706491","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"二宮 康晴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"706492","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田中 薫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"706493","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"根井 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"706494","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"王 冰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"706495","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"conference object","resourceuri":"http://purl.org/coar/resource_type/c_c94f"}]},"item_title":"High Radiosensitivity of  Bone Marrow Cells in GFP Transgenic Mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High Radiosensitivity of  Bone Marrow Cells in GFP Transgenic Mice"}]},"item_type_id":"10005","owner":"1","path":["28"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-08-06"},"publish_date":"2015-08-06","publish_status":"0","recid":"71778","relation_version_is_last":true,"title":["High Radiosensitivity of  Bone Marrow Cells in GFP Transgenic Mice"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T19:47:31.233701+00:00"}